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TRKA antibody (Extracellular)

KO Validated NTRK1 Reactivity: Rat WB, IHC, IF (cc), IF, IC, LCI Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7043385
  • Target See all TRKA (NTRK1) Antibodies
    TRKA (NTRK1) (Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))
    Binding Specificity
    • 40
    • 20
    • 19
    • 18
    • 16
    • 15
    • 15
    • 11
    • 11
    • 8
    • 8
    • 8
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 342-356, Extracellular
    Reactivity
    • 225
    • 144
    • 134
    • 15
    • 3
    • 3
    • 1
    • 1
    Rat
    Host
    • 237
    • 14
    • 3
    • 2
    • 1
    Rabbit
    Clonality
    • 231
    • 25
    • 1
    Polyclonal
    Conjugate
    • 97
    • 17
    • 14
    • 12
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This TRKA antibody is un-conjugated
    Application
    • 176
    • 96
    • 79
    • 78
    • 49
    • 37
    • 29
    • 18
    • 16
    • 14
    • 8
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)
    Purpose
    A Rabbit Polyclonal Antibody to TrkA
    Specificity
    2nd extracellular immunoglobulin-like domain
    Cross-Reactivity
    Human, Mouse, Rat
    Predicted Reactivity
    Mouse - identical, human - 13,14 amino acid residues identical
    Characteristics
    Anti-TrkA (extracellular) Antibody (ABIN7043385, ABIN7044771 and ABIN7044772)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and indirect flow cytometry applications. It has been designed to recognize TrkA from mouse, rat, and human samples.
    Purification
    Affinity purified on immobilized antigen.
    Grade
    KO Validated
    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)ETMRHG(S)LRLNQPTH, corresponding to amino acid residues 342-356 of rat TrkA

    Isotype
    IgG
    Top Product
    Discover our top product NTRK1 Primary Antibody
  • Application Notes

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100-1:1000

    Application Dilutions Western blot wb: 1:200

    Comment

    Cited Application: IHC|ICC

    Negative Control: BLP-NT018

    Blocking Peptide: BLP-NT018

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4
    Storage
    4 °C,-20 °C
    Storage Comment

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    TRKA (NTRK1) (Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))
    Alternative Name
    NTRK1 (NTRK1 Products)
    Background
    High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, NTRK1,NGF was the first neurotrophin to be discovered more than 50 years ago. Three other proteins have been added to the list: BDNF, NT-3 and NT-4 (NT-4/5). These neurotrophins bind two groups of receptors. The p75NTR receptor is common to all four neurotrophins and is a member of the tumor necrosis factor receptor family. The tropomyosin-related kinase (TrK) receptors are receptor tyrosine kinases (RTKs) and three receptors form this family: TrkA, TrkB, and TrkC1.As mentioned, the p75NTR receptor binds to all neurotrophins with similar affinities while the Trk receptors display selectivity for the neurotrophins. TrkA is activated by NGF binding, TrkB by that of BDNF and NT-4, while TrkC is stimulated by the binding of NT-31.All three Trk receptors are highly expressed in the mammalian brain in very distinct regions and are also expressed in the peripheral nervous system2-4. Cholinergic neurons in the basal forebrain exclusively express TrkA. All three Trk receptors are expressed in the hippocampus. Motor and sensory neurons in the peripheral nervous system express Trk receptors. Interestingly, while Trk receptors are not essential for development, knockout mice die shortly after birth. Indeed, TrkA-deficient mice demonstrate a significant decrease in cholinergic and sympathetic neurons in the trigeminal, superior cervical and dorsal root ganglia5-7.Trk receptors are type I membrane proteins (i.e. span the membrane with one transmembrane region) and have many motifs in the extracellular region, including cell-adhesion domains, three tandem leucine rich motifs flanked by two clusters of cysteines. In the membrane proximal region of the receptor there are also two immunoglobulin-like domains8. The intracellular region of the protein contains the kinase domain, responsible for activating downstream effectors as well as many adaptor site regions important for the binding/interaction of signaling molecules.The binding of neurotrophins to Trk receptors promotes receptor dimerization resulting in kinase activation. Activated Trk receptors then phosphorylate a cascade of signaling molecules including the Ras/ERK, PI3K/Akt pathways and PLC-γ1. Activated Trk receptors also create internal docking sites for other signaling adaptor proteins to bind8.Splice variants of TrkA, TrkB and TrkC have been observed. These splice isoforms are mainly affected in the tyrosine kinase domain of the receptor lying in the cytoplasm. Endocytosis is an important signaling trait of Trk receptors. Following neurotrophin binding to the Trk receptor, the receptor complex is then internalized via endocytosis in order to terminate signaling. However, in the axonal compartment of neurons, the internalization process of the neurotrophin complexed to the receptor is part of the signaling process and is important for activating transcription processes in the nucleus9,10.

    Alternative names: TrkA, High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, NTRK1
    Gene ID
    59109
    NCBI Accession
    NM_002529
    UniProt
    P35739
    Pathways
    RTK Signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process
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