TRKA antibody (pTyr681, Tyr680)

Catalog No. ABIN6256787

This Rabbit Polyclonal antibody specifically detects TRKA in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.It has been mentioned in 1 publication

Quick Overview for TRKA antibody (pTyr681, Tyr680) (ABIN6256787)

Target: TRKA (NTRK1) (Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRKA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-TRKA Antibody

Binding Specificity: pTyr681, Tyr680

Specificity: Phospho-TrkA (Tyr680+Tyr681) Antibody detects endogenous levels of TrkA only when phosphorylated at Tyrosine 680+Tyrosine 681.

Predicted Reactivity: Pig,Zebrafish,Bovine,Sheep,Rabbit,Dog,Chicken

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human TrkA around the phosphorylation site of Tyr680+Tyr681.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-TRKA antibody (ABIN6256787)

Liu, Geng, Zhang, Wang, Zhang, Duan, Zhang: "Oligo-Porphyran Ameliorates Neurobehavioral Deficits in Parkinsonian Mice by Regulating the PI3K/Akt/Bcl-2 Pathway." in: Marine drugs, Vol. 16, Issue 3, (2018) (PubMed).

Target Details for TRKA

Target: TRKA (NTRK1) (Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))

Alternative Name: NTRK1

Background:

Description: Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand (PubMed:1850821, PubMed:1849459, PubMed:1281417, PubMed:8325889, PubMed:15488758, PubMed:17196528, PubMed:27445338). Can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival (By similarity). Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed:1281417). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.

Gene: NTRK1

Molecular Weight: 87kDa

Gene ID: 4914

UniProt: P04629

Pathways: RTK Signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process