Neurotrophin 4 antibody (Intracellular)

Catalog No. ABIN7043393

Product Details anti-Neurotrophin 4 Antibody

Target: Neurotrophin 4 (NTF4)

Binding Specificity: AA 43-52, AA 123-132, Intracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neurotrophin 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Neurotrophin 4

Specificity: Intracellular

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical

Characteristics: Anti-Neurotrophin 4 Antibody is directed against an epitope of human Neurotrophin 4. Anti-Neurotrophin 4 Antibody (ABIN7043393 and ABIN7044750) can be used in western blot and immunohistochemistry applications. It has been designed to recognize from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: LGEVPAAGGSPL(C), corresponding to amino acid residues 43-52 of mature human NT-4 (residues 123-132 of the precursor NT-4) 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IFC

Negative Control: (ABIN7566010)

Blocking Peptide: (ABIN7566010)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Neurotrophin 4

Target: Neurotrophin 4 (NTF4)

Alternative Name: NTF4 (NTF4 Products)

Background: NTF4, Neutrophic factor 4, Neurotrophin 5, NT-5, NTF5, Neutrophic factor 5, NT 4/5,The neurotrophins ("neuro" meaning nerve and "trophe" meaning nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and PNS.1 Neurotrophin-4 (NT-4) is expressed in neurons of the superior cervical, stellate and celiac ganglion,2 T-cells3 and is synthesized by keratinocytes.4 The structural hallmark of all neurotrophins is the characteristic arrangement of the disulfide bridges, known as the cysteine knot, which has been found in other growth factors such as PDGF.5 The rat and human forms of NT-4 are 96 % homologous. NT-4 has been shown to promote dendritic outgrowth and calcium currents in cultured mesencephalic dopamine neurons,6 to promote growth and remodeling of adult motor neuron innervation,7 to be anterograde survival factors for postsynaptic cells8 and to protect against apoptotic neuronal death.9 The biological effects of NT-4 are mediated by two receptors: TrkB, which is specific for NT-4 and BDNF, and p75NTR, which binds all the neurotrophins.10

Alternative names: Neurotrophin 4 (NT-4), NTF4, Neutrophic factor 4, Neurotrophin 5, NT-5, NTF5, Neutrophic factor 5, NT 4/5

Gene ID: 16485

NCBI Accession: NM_006179

UniProt: P34130

Pathways: RTK Signaling