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NUCB2 antibody (Intracellular)

NUCB2 Reactivity: Rat WB, IHC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7043396
  • Target See all NUCB2 Antibodies
    NUCB2 (Nucleobindin 2 (NUCB2))
    Binding Specificity
    • 6
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 30-44, Intracellular
    Reactivity
    • 32
    • 10
    • 6
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Rat
    Host
    • 37
    • 1
    Rabbit
    Clonality
    • 37
    • 1
    Polyclonal
    Conjugate
    • 19
    • 7
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NUCB2 antibody is un-conjugated
    Application
    • 24
    • 13
    • 9
    • 5
    • 4
    • 4
    • 3
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC)
    Purpose
    A Rabbit Polyclonal Antibody to Nucleobindin-2/Nesfatin-1
    Specificity
    Intracellular
    Cross-Reactivity
    Human, Mouse, Rat
    Predicted Reactivity
    human -11,Mouse -13,15 amino acid residues identical
    Characteristics
    Anti-Nucleobindin-2/Nesfatin-1 Antibody (ABIN7043396, ABIN7044216 and ABIN7044217) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize Nucleobindin-2/Nesfatin-1 from human, mouse, and rat samples.
    Purification
    Affinity purified on immobilized antigen.
    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)DKTKVHNVEPVESAR, corresponding to amino acid residues 30-44 of rat Nucleobindin-2/Nesfatin-1

    Isotype
    IgG
    Top Product
    Discover our top product NUCB2 Primary Antibody
  • Application Notes

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1200

    Application Dilutions Western blot wb: 1:200-1:400

    Comment

    Negative Control: (ABIN7236159)

    Blocking Peptide: (ABIN7236159)

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4
    Storage
    4 °C,-20 °C
    Storage Comment

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    NUCB2 (Nucleobindin 2 (NUCB2))
    Alternative Name
    NUCB2 (NUCB2 Products)
    Background
    NUCB2, NEFA, Prepronesfatin, DNA-binding protein NEFA, Gastric cancer antigen Zg4, HEL-S-109,Nucleobindin contains multiple functional domains including a signal peptide on the N-terminal side, a leucine/isoleucine rich region, a DNA binding domain and a putative nuclear targeting signal. The second half of the protein contains two Ca2+-EF-hand motifs and a leucine zipper motif in the C-terminal region. To date, two nucleobindins have been identified, namely nucleobindin 1 and nucleobindin 2.Nucleobindin 2 (NUCB2) can be found on the plasma membrane and in the cytoplasm. Putative post-translational processing of NUCB2 by the enzyme pro-hormone convertase (PC)-1/3 results in nesfatin-1, nesfatin-2 and nesfatin-3. So far, a biological activity has only been demonstrated for nesfatin-1 and its fragment, nesfatin-124-531.The gene Nesfatin/NUCB2 encoding the protein is expressed in the appetite control hypothalamic nuclei such as the paraventricular nucleus (PVN), the supraoptic nucleus, the lateral hypothalamic area and the zona increta in rats. Nefstain-1 immunoreactivity is also detected in the brain stem nuclei such as the nucleus of the solitary tract and the dorsal nucleus of the vagus.Nesfatin-1 has a substantial anorexigenic effect through the selective reduction of nesfatin/NUCB mRNA expression in the PVN of rats under starved conditions. Refeeding of rats was found to restore Nesfatin-1 reduction. A similar effect was not observed in other hypothalamic nuclei2. In humans, Nesfatin-1 levels in the saliva and serum of epilepsy patients are reduced with the use of anti-epileptic medication, but are still higher than the amounts in normal subjects. In addition, nesfatin-1 levels are decreased in human subjects with type-2 diabetes and it has been hypothesized that the observed decrement might be associated with diabetic hyperphagia3.

    Alternative names: Nucleobindin-2/Nesfatin-1, NUCB2, NEFA, Prepronesfatin, DNA-binding protein NEFA, Gastric cancer antigen Zg4, HEL-S-109
    Gene ID
    59295
    NCBI Accession
    NM_005013
    UniProt
    Q9JI85
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