ORAI1 antibody (Extracellular) (Atto 633)

Catalog No. ABIN7043411

Product Details anti-ORAI1 Antibody (Atto 633)

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Binding Specificity: Extracellular

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ORAI1 antibody is conjugated to Atto 633

Application: Flow Cytometry (FACS)

Cross-Reactivity (Details): Recognizes only human Orai1.

Characteristics: Mouse Anti-Human Orai1 (extracellular) Antibody (ABIN7043409 and ABIN7044554)) is a highly specific monoclonal antibody directed against an extracellular epitope of the human Orai1 channel. The antibody can be used in western blot, immunocytochemical and indirect flow cytometry applications and was designed to recognize Orai1 from human samples only. \nMouse Anti-Human Orai1 (extracellular)-ATTO Fluor-633 Antibody (#ABIN7043411) is directly labeled with an ATTO-633 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. ATTO 633 has a maximum absorption at 629 nm and a maximum fluorescence at 657 nm. The fluorescence is excited most efficiently in the range 610 to 645 nm. This label is analogous to the well-known dyes Alexa 647, Alexa 633 and Cy5. Mouse Anti-Human Orai1 (extracellular)-ATTO Fluor-633 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified from cultured hybridoma medium.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Synthetic mapping to an extracellular sequence of human Orai1

Isotype: IgM

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ORAI1

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Alternative Name: Orai1 (ORAI1 Products)

Background: Alternative names: Orai1, TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1

Gene ID: 84876

NCBI Accession: NM_032790

UniProt: Q96D31

Pathways: TCR Signaling, BCR Signaling