ORAI1 antibody (Extracellular) (Atto 633)

Catalog No. ABIN7043411

The Mouse Monoclonal anti-ORAI1 antibody has been validated for FACS and LCI. It is suitable to detect ORAI1 in samples from Human.

Quick Overview for ORAI1 antibody (Extracellular) (Atto 633) (ABIN7043411)

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ORAI1 antibody is conjugated to Atto 633

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Clone: 3F11-D10-B9

Product Details anti-ORAI1 Antibody (Atto 633)

Binding Specificity: Extracellular

Purpose: A Mouse Monoclonal Antibody to Human Orai1 Channel Conjugated to the Fluorescent Dye ATTO-633

Specificity: Extracellular

Cross-Reactivity (Details): Recognizes only human Orai1.

Predicted Reactivity: Recognizes Orai1 from human samples only

Characteristics: Mouse Anti-Human Orai1 (extracellular) Antibody (ABIN7043409 and ABIN7044554) is a highly specific monoclonal antibody directed against an extracellular epitope of the human Orai1 channel. The antibody can be used in western blot, immunocytochemical and indirect flow cytometry applications and was designed to recognize Orai1 from human samples only. \nMouse Anti-Human Orai1 (extracellular)-ATTO Fluor-633 Antibody (ABIN7043411) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. ATTO 633 has a maximum absorption at 629 nm and a maximum fluorescence at 657 nm. The fluorescence is excited most efficiently in the range 610 to 645 nm. This label is analogous to the well-known dyes Alexa 647, Alexa 633 and Cy5. Mouse Anti-Human Orai1 (extracellular)-ATTO Fluor-633 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Synthetic mapping to an extracellular sequence of human Orai1

Isotype: IgM

Application Details

Application Notes:

Antigen preadsorption control: NA

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ORAI1

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Alternative Name: ORAI1

Background: TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1,Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling intracellular Ca2+ level influx either by the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores, has become of great interest.Recently, several key players of the store operated complex have been identified3. Orai1 (also known as CRACM1) acts as the store operated Ca2+ channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca2+ sensor3,4. The formation of functional channels requires the presence of both Orai1 and STIM1 proteins working as a complex and involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum4-6. TRPC1, a member of the transient receptor potential family was also suggested to act as a player in the SOC complex7. In T-cells, Ca2+ entry following activation by antigen-receptor engagement occurs solely through CRAC channels where Orai1 constitutes the pore forming subunit3,8.Orai1 is a plasma membrane protein with four potential transmembrane domains and intracellular N- and C-terminus. In addition, two mammalian homologs to Orai1 have been identified, Orai2 and Orai33,9. All three, Orai1 Orai2 and Orai3, are capable of forming store operated channels with different magnitudes9.

Alternative names: Orai1, TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1

Gene ID: 84876

NCBI Accession: NM_032790

UniProt: Q63633

Pathways: TCR Signaling, BCR Signaling