ORAI1 antibody (2nd Extracellular Loop)
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- Target See all ORAI1 Antibodies
- ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))
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Binding Specificity
- 2nd Extracellular Loop, AA 200-212
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Reactivity
- Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ORAI1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunocytochemistry (ICC)
- Cross-Reactivity (Details)
- Will not recognize human Orai1.
- Characteristics
- Anti-Orai1 (extracellular) Antibody (ABIN7043408, ABIN7044024 and ABIN7044025)) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and indirect flow cytometry applications. It has been designed to recognize the Orai1 channel from mouse and rat samples.
- Purification
- Affinity purified on immobilized antigen.
- Immunogen
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)KFLPLKRQAGQPS, corresponding to amino acid residues 200-212 of mouse Orai1
- Isotype
- IgG
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
- Concentration
- 0.65 mg/mL
- Buffer
- Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- RT,4 °C,-20 °C
- Storage Comment
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Target
- ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))
- Alternative Name
- Orai1 (ORAI1 Products)
- Synonyms
- CRACM1 antibody, ORAT1 antibody, TMEM142A antibody, RGD1311873 antibody, D730049H07Rik antibody, Tmem142a antibody, orai-1 antibody, im:7146264 antibody, orai1 antibody, tmem142a antibody, zgc:109721 antibody, orai2 antibody, ORAI1 antibody, LOC100218031 antibody, ORAI calcium release-activated calcium modulator 1 antibody, ORAI calcium release-activated calcium modulator 1b antibody, ORAI calcium release-activated calcium modulator 1 S homeolog antibody, ORAI1 antibody, Orai1 antibody, orai1b antibody, orai1.S antibody, orai1 antibody
- Background
- Alternative names: Orai1, TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1
- Gene ID
- 109305
- NCBI Accession
- NM_032790
- UniProt
- Q8BWG9
- Pathways
- TCR Signaling, BCR Signaling
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