Periaxin antibody (Intracellular)

Catalog No. ABIN7043432

Product Details anti-Periaxin Antibody

Target: Periaxin (PRX)

Binding Specificity: AA 155-170, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Periaxin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Periaxin

Specificity: Intracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Specific to isoform 1 also known as L-Periaxin (will not recognize isoform 2 known as S-Periaxin).

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Periaxin Antibody (ABIN7043432, ABIN7044712 and ABIN7044713) is a highly specific antibody directed against an epitope of rat PRX. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize PRX from mouse, rat and human samples. The antibody specifically recognizes L-Periaxin. It does not recognize S-Periaxin.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DVEFSFPKFSRLRR, corresponding to amino acid residues 155-170 of rat PRX

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:600

Application Dilutions Western blot wb: 1:600

Comment:

Negative Control: (ABIN7236245)

Blocking Peptide: (ABIN7236245)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Periaxin

Target: Periaxin (PRX)

Alternative Name: PRX (PRX Products)

Background: PRX, CMT4F,Periaxin protein is a membrane-associated protein expressed in myelinating Schwann cells. It is required for the stable maintenance and development of a normal myelin sheath in the peripheral nervous system. It is also a member of cytoskeletal complexes in lens fibers where it plays an important role in maturation, packing, and membrane organization of lens fiber cell1,2. The periaxin gene (PRX) encodes two protein isoforms, L-periaxin and S-periaxin generated by alternative mRNA splicing. These proteins contain N-terminal PDZ domains (PSD-95/Discs-large/ZO-1) important for organizing protein-protein interactions.L-periaxin is a component of the dystroglycan-dystrophin-related protein-2 complex (DRP2) linking the Schwann cell cytoskeleton to the extracellular matrix. Its location changes during myelination, at first the protein is localized to the adaxonal plasma membrane and later, in the abaxonal plasma membrane. S-periaxin is distributed in a uniform manner in the cytoplasm and the nucleus of the Schwann cell1-3. Mutations in the Periaxin gene are known to cause autosomal recessive demyelinating in Type 4F Charcot-Marie-Tooth (CMT4F) and Dejerine-Sottas disease3.Periaxin knockout mice develop normally myelinated peripheral nervous system at first but develop a late onset demyelinating peripheral neuropathy1.

Alternative names: Periaxin, PRX, CMT4F

Gene ID: 78960

NCBI Accession: NM_020956

UniProt: Q63425