Plexin C1 antibody (Extracellular)

Catalog No. ABIN7043439

Product Details anti-Plexin C1 Antibody

Target: Plexin C1 (PLXNC1)

Binding Specificity: AA 860-874, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Plexin C1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal antibody to Plexin-C1 (extracellular)

Specificity: Extracellular, N-terminus.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical Rat - 14 out of 15 amino acid residues identical

Characteristics: A rabbit polyclonal antibody to Plexin-C1 (extracellular)

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Peptide (C)EVKIQKENDNFNISK, corresponding to amino acid residues 860 - 874 of human Plexin-C1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-PR091

Blocking Peptide: BLP-PR091

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Plexin C1

Target: Plexin C1 (PLXNC1)

Alternative Name: PLXNC1 (PLXNC1 Products)

Background: Virus-Encoded Semaphorin Protein Receptor, VESPR, CD232, PLXNC1,Plexin-C1 also known as Virus-encoded semaphorin protein receptor, is a ~210 kDa type I transmembrane receptor that regulates cytoskeletal remodeling and adhesion in human melanocytes.It belongs to the family of Plexin receptors, these are type I transmembrane proteins (A-D) that share a highly conserved cytoplasmic domain and an intracellular region containing an intrinsic Ras-GTPase activating (GAP) domain. Plexin receptors bind semaphorins: a class of secreted and membrane proteins 1. The natural ligand of Plexin C1 is Semaphorin -7A (#ASR-077).Plexin-C1 is highly expressed in melanocytes, neurons, neutrophils, dendritic cells and pre-osteoblasts and it has a complex effect on migration of melanocytes and melanoma, that depends on the phase of progression of this type of cancer 2,3. It also has additional roles in the partitioning of paraventricular and supraoptic neurons in the hypothalamus, in the development of inflamation and in inhibition of neutrophils phagocytosis 3,4.

Alternative names: Virus-Encoded Semaphorin Protein Receptor, VESPR, CD232, PLXNC1

Gene ID: 10154

NCBI Accession: NM_005761

UniProt: O60486