KCNK13 antibody (Extracellular)

Catalog No. ABIN7043444

Product Details anti-KCNK13 Antibody

Target: KCNK13 (Potassium Channel Subfamily K Member 13 (KCNK13))

Binding Specificity: AA 54-69, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNK13 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Live Cell Imaging (LCI), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to KCNK13 Channel

Specificity: 1st extracellular loop

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 15,16 amino acid residues identical

Characteristics: Anti-KCNK13 (THIK-1) (extracellular) Antibody is directed against an epitope in the 1st extracellular loop of the rat KCNK13 channel. Anti-KCNK13 (THIK-1) (extracellular) Antibody (ABIN7043444, ABIN7045019 and ABIN7045020)) can be used in western blot and immunohistochemical applications, and recognizes KCNK13 channel from mouse and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EERLANFSRGHNLSRE, corresponding to amino acid residues 54-69 of rat KCNK13 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:50-1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-PC121

Blocking Peptide: BLP-PC121

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNK13

Target: KCNK13 (Potassium Channel Subfamily K Member 13 (KCNK13))

Alternative Name: KCNK13 (KCNK13 Products)

Background: Potassium channel subfamily K member 13, Tandem pore domain halothane-inhibited K+ channel 1, K2P13.1,KCNK13 (also named Tandem pore domain halotane inhibited K+ channel, THIK -1 or K2P13.1) is a member of the 2-pore (2P) domain K+ channels family that currently includes 16 members. These channels show little time- or voltage-dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.1The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. It has been proposed that the functional channel unit is a dimer.K2P channels are regulated by diverse physical and chemical stimuli including temperature, pH , mechanical stretch, inhalation anesthetics, etc. but are insensitive to classical K+ channel blockers.The KCNK13 channel is activated by arachidonic acid and inhibited by the volatile anesthetic halothane. The channel is relatively widely expressed in peripheral tissues such as kidney, heart, lung and liver but it is also expressed in brain.The physiological function of the KCNK13 channel is not clear but an involvement in the physiological response to reduced oxygen levels (hypoxia) has been proposed.2

Alternative names: KCNK13 (THIK-1), Potassium channel subfamily K member 13, Tandem pore domain halothane-inhibited K+ channel 1, K2P13.1

Gene ID: 64120

NCBI Accession: NM_022054

UniProt: Q9ERS0