KCNT1 antibody (Intracellular)

Catalog No. ABIN7043457

Product Details anti-KCNT1 Antibody

Target: KCNT1 (Potassium Channel, Subfamily T, Member 1 (KCNT1))

Binding Specificity: AA 619-631, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to KCNT1 (Slack)

Specificity: Intracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Unlikely to recognize human samples.

Predicted Reactivity: Mouse - 12,13 amino acid residues identical

Characteristics: Anti-KCNT1 (Slack) Antibody is directed against an epitope located at the intracellular C-terminal domain of rat KCNT1. Anti-KCNT1 (Slack) Antibody (ABIN7043457, ABIN7045025 and ABIN7045026)) can be used in western blot applications, and was designed to recognize KCNT1 from rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KQEEKQNRRGLAG, corresponding to amino acid residues 619-631 of rat Slack

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-PC124

Blocking Peptide: BLP-PC124

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNT1

Target: KCNT1 (Potassium Channel, Subfamily T, Member 1 (KCNT1))

Alternative Name: KCNT1 (KCNT1 Products)

Background: KCa4.1, KNa1.1, Slo2.2 Na+-activated K+ channel, Potassium channel subfamily T member 1,KCa4.1 (Na+-activated K+ channel, Slack, Slo2.2, KCNT1) is a member of a subgroup within the Ca2+ activated K+ channel family. The Slack (Sequence like a Ca2+-activated K+ channel) and Slick (Sequence like an intermediate conductance K+ channel, KCNT2) genes, which encode Na+-activated K+ (KNa) channels, are expressed at high levels in neurons in several areas of the nervous system1.Although KCa4.1 is functionally a Na+-activated K+ channel, it is termed KCa by the IUPHAR nomenclature, due to its sequence homology to other KCa channels.The family's protein topology consists of six transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels, the functional unit for KCa4.1 channels is composed of four subunits that can assemble as either homo- or heteromers with KCNT2 (Slick). KCa4.1 and Slick subunits coassemble to form heteromeric channels that differ from the homomers in their unitary conductance, kinetic behavior, subcellular localization, and response to activation of protein kinase C. Heteromer formation requires the N-terminal domain of Slack-B, one of the alternative splice variants of the KCa4.1 channel. This cytoplasmic N-terminal domain of Slack-B also facilitates the localization of heteromeric KNa channels to the plasma membrane2.K+ channels sensitive to intracellular Na+, termed KNa channels, shape neuronal excitability. KNa channels contribute to the adaptation of firing rates and to slow after-hyperpolarizations that follow repetitive firing. In certain neurons, they also appear to be activated by Na+ influx accompanying single action potentials. Their molecular properties also suggest that these channels contribute to the response of neurons to hypoxia1. KCa4.1 channels were shown to be expressed also in dorsal root ganglion (DRG) neurons, where these channels were found to contain a NAD+ binding sequence and to be modulated by this endogenous compound3.

Alternative names: KCNT1 (Slack), KCa4.1, Slo2.2 Na+-activated K+ channel, Potassium channel subfamily T member 1

Gene ID: 60444

NCBI Accession: NM_020822

UniProt: Q9Z258