KCNJ5 antibody (Intracellular)

Catalog No. ABIN7043486

Product Details anti-KCNJ5 Antibody

Target: KCNJ5 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 5 (KCNJ5))

Binding Specificity: AA 6-18, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNJ5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to KCNJ5 (Kir3.4, GIRK4) Channel

Specificity: Intracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human,pig - identical

Characteristics: Anti-KCNJ5 (Kir3.4) Antibody is directed against an epitope of the rat Kir3.4 channel. Anti-KCNJ5 (Kir3.4) Antibody (ABIN7043486, ABIN7044936 and ABIN7044937)) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Kir3.4 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: RNAMNQDMEIGVT(C), corresponding to amino acid residues 6-18 of rat Kir3.4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP

Negative Control: BLP-PC027

Blocking Peptide: BLP-PC027

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNJ5

Target: KCNJ5 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 5 (KCNJ5))

Alternative Name: KCNJ5 (KCNJ5 Products)

Background: GIRK4, G protein-activated inward rectifier potassium channel 4, Cardiac inward rectifier, CIR, KATP-1,Kir3.4 is a member of the G-protein regulated inward-rectifier K+ (GIRK) channel subfamily which is part of an inward-rectifier K+ channel superfamily. The GIRK subfamily comprises four members in mammals (Kir3.1- Kir3.4) that present the common topology of the inward-rectifier superfamily: two transmembrane domains flanking a highly conserved pore region with the N and C-terminus located intracellularly.Kir3.4 and the other Kir3 family subunits can be activated by neurotransmitters and other factors via the activation of G-protein coupled receptors. Binding of the corresponding ligand to the G-protein receptor induces the dissociation of G α-GTP from the Gβγ dimer. The latter directly binds to Kir3 and activates the channel. Kir3.4 expression is largely confined to the heart, where it co-assembles with Kir3.1 to form the prototypical muscarinic-gated K+ channel KACh. Indeed, knockout mice for Kir3.4 showed impaired heart rate following vagal nerve stimulation.A peptide toxin originating from the Apis mellifera bee venom, Tertiapin (#STT-250) was shown to be a potent blocker of Kir3.4 containing channels (8.6 nM for the Kir3.1/3.4 combination).4

Alternative names: KCNJ5 (Kir3.4), GIRK4, G protein-activated inward rectifier potassium channel 4, Cardiac inward rectifier, CIR, KATP-1

Gene ID: 29713

NCBI Accession: NM_000890

UniProt: P48548

Pathways: Notch Signaling