P2RX1 antibody (Intracellular)

Catalog No. ABIN7043570

Product Details anti-P2RX1 Antibody

Target: P2RX1 (Purinergic Receptor P2X, Ligand Gated Ion Channel 1 (P2RX1))

Binding Specificity: AA 382-399, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P2RX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunochromatography (IC), Immunoprecipitation (IP)

Purpose: A Rabbit Polyclonal Antibody to P2X1 Receptor

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: 18 amino acid residues identical, human - 15,Mouse - 17

Characteristics: Anti-P2X1 Receptor Antibody is directed against an epitope of the rat P2X1 receptor. Anti-P2X1 Receptor Antibody (ABIN7043570, ABIN7045074 and ABIN7045075) can be used in western blot, immunoprecipitation, indirect flow cytometry, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize P2X1 receptor from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (CS)DPVATSSTLGLQENMRTS, corresponding to amino acid residues 382-399 of rat P2X1 receptor 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC|ICC|IFC

Negative Control: (ABIN7236648)

Blocking Peptide: (ABIN7236648)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for P2RX1

Target: P2RX1 (Purinergic Receptor P2X, Ligand Gated Ion Channel 1 (P2RX1))

Alternative Name: P2RX1 (P2RX1 Products)

Background: P2RX1, P2X purinoceptor 1,The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.The structure and function of the P2X receptors, investigated mainly using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.Seven mammalian P2X receptor subtypes (P2X1-P2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N and C-termini two membrane-spanning domains and a large extracellular loop.All P2X receptor subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex.4-9The various P2X receptor subtypes show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4 The P2X1 receptor is present in smooth muscle, cerebellum, dorsal horn spinal neurons, and platelets where it is suggested to play a regulatory role during in vivo homeostasis and thrombosis.3,4,10,11

Alternative names: P2X1 Receptor, P2RX1, P2X purinoceptor 1

Gene ID: 25505

NCBI Accession: NM_002558

UniProt: P47824

Pathways: Positive Regulation of Endopeptidase Activity