P2RY1 antibody (Extracellular) (Atto 488)

Catalog No. ABIN7043585

Product Details anti-P2RY1 Antibody (Atto 488)

Target: P2RY1 (Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1))

Binding Specificity: AA 207-220, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P2RY1 antibody is conjugated to Atto 488

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to P2Y1 Receptor Conjugated to the Fluorescent Dye ATTO-488

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical, mouse - 12,14 amino acid residues identical

Characteristics: Anti-P2Y1 Receptor (extracellular) Antibody (ABIN7043584, ABIN7045094 and ABIN7045095) is a highly specific antibody directed against an extracellular epitope of the human P2RY1. The antibody can be used in western blot, immunohistochemistry, indirect flow cytometry and live cell imaging applications. It has been designed to recognize P2RY1 from mouse, rat and human samples. \nAnti-P2Y1 Receptor (extracellular)-ATTO Fluor-488 Antibody (ABIN7043585) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-P2Y1 Receptor (extracellular)-ATTO Fluor-488 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: SDEYLRSYFIYSMC, corresponding to amino acid residues 207-220 of human P2RY1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:80

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582041)

Blocking Peptide: (ABIN7236684)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for P2RY1

Target: P2RY1 (Purinergic Receptor P2Y, G-Protein Coupled, 1 (P2RY1))

Alternative Name: P2RY1 (P2RY1 Products)

Background: P2Y purinoceptor 1, P2RY1,The P2Y receptors belong to the G-protein coupled receptors superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiologic responses, among them, cardiac function, platelet aggregation and SMC proliferation.1The mammalian P2Y family currently includes eight functional receptors, P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.2-4P2Y1 receptor is abundantly expressed in a number of tissues. In platelets, P2Y1 receptor is co-expressed with P2Y12 receptor, responsible for the initial shape change, aggregation, and rise in intracellular Ca2+ upon activation in response to ADP.5

Alternative names: P2Y1 Receptor, P2Y purinoceptor 1, P2RY1

Gene ID: 5028

NCBI Accession: NM_002563

UniProt: P47900

Pathways: Regulation of Carbohydrate Metabolic Process, Feeding Behaviour