P2RY6 antibody (Extracellular)

Catalog No. ABIN7043591

Product Details anti-P2RY6 Antibody

Target: P2RY6 (Pyrimidinergic Receptor P2Y, G-Protein Coupled, 6 (P2RY6))

Binding Specificity: AA 172-186, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P2RY6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to P2Y6 Receptor

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,15 amino acid residues identical, human - 12,rat - 14

Characteristics: Anti-P2Y6 Receptor (extracellular) Antibody (ABIN7043591, ABIN7045140 and ABIN7045141) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and indirect live cell flow cytometry applications. It has been designed to recognize P2RY6 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RNRTV(S)YDLSPPILS, corresponding to amino acid residues 172 - 186 of mouse P2RY6

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Negative Control: (ABIN7236699)

Blocking Peptide: (ABIN7236699)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for P2RY6

Target: P2RY6 (Pyrimidinergic Receptor P2Y, G-Protein Coupled, 6 (P2RY6))

Alternative Name: P2RY6 (P2RY6 Products)

Background: P2Y purinoceptor 6, P2RY6,The nucleotide receptor P2Y6 binds UDP, and is a member of the G-protein coupled receptor superfamily1,3.P2Y6 agonists induce an inositol phosphate/Ca2+ response that is insensitive to pertussis toxin inhibition. P2Y6 is coupled to phospholipase C via Gq, and is also coupled to inhibition of N-type Ca2+ and M-type K+ channels1,2.P2Y6 mRNA is expressed in various rat tissues, including lung, stomach, intestine, spleen, heart, and aorta. P2Y6 was reported to mediate contractions in human cerebral arteries4.

Alternative names: P2Y6 Receptor, P2Y purinoceptor 6, P2RY6

Gene ID: 233571

NCBI Accession: NM_001277204

UniProt: Q9ERK9

Pathways: Smooth Muscle Cell Migration