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KCNN3 antibody (Intracellular)

KCNN3 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7043633
  • Target See all KCNN3 Antibodies
    KCNN3 (Small Conductance Calcium-Activated Potassium Channel Protein 3 (KCNN3))
    Binding Specificity
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    AA 659-674, Intracellular
    Reactivity
    • 16
    • 7
    • 7
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 15
    • 1
    Rabbit
    Clonality
    • 16
    Polyclonal
    Conjugate
    • 12
    • 1
    • 1
    • 1
    • 1
    This KCNN3 antibody is un-conjugated
    Application
    • 10
    • 8
    • 7
    • 5
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Purpose
    A Rabbit Polyclonal Antibody to KCNN3 (KCa2.3, SK3) Channel
    Specificity
    Intracellular, C-terminal domain
    Cross-Reactivity
    Human, Mouse, Rat
    Predicted Reactivity
    Rat,mouse,pig - identical
    Characteristics
    Anti-KCNN3 (KCa2.3, SK3) (C-term) Antibody (ABIN7043633, ABIN7044996 and ABIN7044997)) is a highly specific antibody directed against an epitope of the human KCNN3 channel. The antibody can be used in western blot analysis. It has been designed to recognize KCNN3 from human, rat, and mouse samples.
    Purification
    Affinity purified on immobilized antigen.
    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)KQIGSLESKLEHLTAS, corresponding to amino acid residues 659-674 of human KCNN3 

    Isotype
    IgG
    Top Product
    Discover our top product KCNN3 Primary Antibody
  • Application Notes

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

    Application Dilutions Western blot wb: 1:200

    Comment

    Cited Application: IP|IHC|ICC

    Negative Control: BLP-PC103

    Blocking Peptide: BLP-PC103

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    0.2 mL double distilled water (DDW).
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4
    Storage
    4 °C,-20 °C
    Storage Comment

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    KCNN3 (Small Conductance Calcium-Activated Potassium Channel Protein 3 (KCNN3))
    Alternative Name
    KCNN3 (KCNN3 Products)
    Background
    Small conductance calcium-activated potassium channel protein 3, SKCa3,KCa2.3 (KCNN3, SK3) is a member of the Ca2+-activated K+ channel family with small conductance that includes KCa2.1 (KCNN1, SK1) and KCa2.2 (KCNN2, SK2). The channel is voltage insensitive and is activated by intracellular Ca2+ in the submicromolar range. It has, though, a similar topology to that of voltage-dependent K+ channels (KV channels), that is six transmembrane domains and intracellular N- and C-termini. The functional channel of all the KCa2 family members is a multimeric protein composed of four pore-forming subunits.KCa2 channels are extremely sensitive to the levels of intracellular Ca2+ and concentrations as low as 300-700 nM can open the channels very rapidly (5-15 ms). Hence, the KCa2 channels are highly sensitive and fast Ca2+ sensors resembling other known Ca2+-binding proteins. This type of Ca2+-dependent activation is achieved by the constitutive binding of the KCa2 channels to calmodulin, a highly expressed Ca2+-binding protein via a calmodulin-binding domain situated at the cytoplasmic C-terminus.Pharmacologically, the KCa2 channels are the only known targets of the bee venom toxin Apamin, with KCa2.1 being the least sensitive, KCa2.2 the most sensitive and KCa2.3 showing intermediate sensitivity.KCa2.3 is predominantly expressed in the nervous system although expression in endothelial cells, heart and liver have been described.KCa2.3 is known to be involved in the regulation of neuronal excitability. They do so mainly via a phenomenon known as after hyperpolarization in which KCa2 channels open in response to increased intracellular Ca2+ concentrations that result from the entry of extracellular Ca2+ through voltage-dependent Ca2+ channels during action potentials. In this way, KCa2 channels effectively form a Ca2+-mediated feedback loop.KCa2.3 is involved in the control of firing rate and subsequent dopamine secretion from midbrain dopaminergic neurons. Since malfunction of these neurons is involved in several pathological disorders such as Parkinson's disease and Schizophrenia, modulators of the KCa2.3 channels have been proposed to be of therapeutic value in these diseases.

    Alternative names: KCNN3 (KCa2.3, SK3), Small conductance Ca2+-activated K+ Channel 3, SKCa3
    Gene ID
    3782
    NCBI Accession
    NM_002249
    UniProt
    Q9UGI6
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