SORL1 antibody (Extracellular)

Catalog No. ABIN7043770

Product Details anti-SORL1 Antibody

Target: SORL1 (Sortilin-Related Receptor, L(DLR Class) A Repeats Containing (SORL1))

Binding Specificity: AA 652-665, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SORL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to SORL1 Receptor

Specificity: Extracellular, N-terminus.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,mouse,rat - 13,14 amino acid residues identical

Characteristics: Anti-SORL1 (extracellular) Antibody (ABIN7043770, ABIN7044728 and ABIN7044729)) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize SORL1 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KRRTPHAT(S)FNGED, corresponding to amino acid residues 652 - 665 of human SORL1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-NR132

Blocking Peptide: BLP-NR132

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SORL1

Target: SORL1 (Sortilin-Related Receptor, L(DLR Class) A Repeats Containing (SORL1))

Alternative Name: SORL1 (SORL1 Products)

Background: Sortilin Related Receptor 1, Low-Density Lipoprotein Receptor relative with 11 Ligand-Binding Repeats, LR11, Sorting Protein-Related Receptor Containing LDLR Class A Repeats, SORLA,Sortilin-related receptor1 (SORL1, also known as LR11 or SORLA) is a receptor involved in intracellular sorting and trafficking of proteins into their respective subcellular compartments1.Direction of proteins into their proper compartments is essential for normal cell function and defects in these pathways are thought to be important factors in the pathogenesis of Alzheimer's disease (AD).The full-length transcript of SORL1 encodes a 250 kDa, membrane-bound protein comprised of multiple functional domains. These include a vacuolar protein sorting 10 (VPS10) domain, five low-density lipoprotein receptor (LDLR) class B repeats, an epidermal growth factor-like (EGF-like) domain, eleven LDLR class A repeats, six fibronectin-type (FN-type) repeats, a transmembrane domain and a cytosolic domain containing recognition motifs for cytosolic adaptors1.SORL1 is widely expressed in the human brain, particularly in neurons of the hippocampus and some nuclei of the brainstem and Purkinje cells, and has slightly weaker expression in neurons of the thalamus and the hypothalamus2. It is also expressed in other tissue types such as the testes, ovaries, thyroid, and lymph nodes3.

Alternative names: Sortilin Related Receptor 1, Low-Density Lipoprotein Receptor relative with 11 Ligand-Binding Repeats, LR11, Sorting Protein-Related Receptor Containing LDLR Class A Repeats, SORLA

Gene ID: 6653

NCBI Accession: NM_003105

UniProt: Q92673

Pathways: Smooth Muscle Cell Migration