SUCNR1 antibody (Extracellular)

Catalog No. ABIN7043783

The Rabbit Polyclonal anti-SUCNR1 antibody has been validated for WB, IHC, FACS and LCI. It is suitable to detect SUCNR1 in samples from Rat.

Quick Overview for SUCNR1 antibody (Extracellular) (ABIN7043783)

Target: SUCNR1 (Succinate Receptor 1 (SUCNR1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUCNR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-SUCNR1 Antibody

Binding Specificity: AA 248-262, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Succinate Receptor 1

Specificity: 3rd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: 15 amino acid residues identical,Mouse - 14, human - 12

Characteristics: Anti-GPR91 (SUCNR1) (extracellular) Antibody (ABIN7043783, ABIN7045308 and ABIN7045309) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and live cell flow cytometry. It has been designed to recognize GPR91 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RNVRIASRLDSWPQG, corresponding to amino acid residues 248-262 of rat GPR91/Succinate Receptor 1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IFC

Negative Control: (ABIN7237300)

Blocking Peptide: (ABIN7237300)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SUCNR1

Target: SUCNR1 (Succinate Receptor 1 (SUCNR1))

Alternative Name: SUCNR1

Background: G-protein coupled receptor 91, Succinate receptor 1,GPR91 (Succinate Receptor 1), a member of the GPCR superfamily, is specifically activated by extracellular succinate outside the tricarboxylic acid cycle, resulting in activation of paracrine and endocrine effectors. Activation of GPR91 is associated with several diseases, including hypertension and diabetes1,2.GPR91 was shown to regulate normal retinal vascularization, proliferative ischemic retinopathy and cortical revascularization post-ischemia. Activation of GPR91 influences different tissues such as kidney, heart, retina, white adipose tissue, and liver.Through the activation of GPR91, succinate affects motility, migration, and growth of dendritic cells. In addition, the receptor directly promotes chemotaxis and potentiates activation initiated by Toll-like receptor agonists in dendritic cells1-3.In the heart GPR91 mediates succinate-induced cardiomyocyte death. It was demonstrated that upholding levels of serum succinate can cause cardiac hypertrophy through the activation of the GPR911,2.

Alternative names: GPR91 (SUCNR1), G-protein coupled receptor 91, Succinate receptor 1

Gene ID: 408199

NCBI Accession: NM_033050

UniProt: Q6IYF9