Synaptophysin antibody (IntravesicularAA 178-190)

Catalog No. ABIN7043790

Product Details anti-Synaptophysin Antibody

Target: Synaptophysin (SYP)

Binding Specificity: IntravesicularAA 178-190

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This Synaptophysin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Guinea Pig Polyclonal Antibody to Synaptophysin

Specificity: 2nd intravesicular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse,human - 12 out of 13 amino acid residues identical

Characteristics: A Guinea Pig Polyclonal Antibody to Synaptophysin.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CRQTGNT(S)KELRD, corresponding to amino acid residues 178-190 of rat Synaptophysin

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7237323)

Blocking Peptide: (ABIN7237323)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Synaptophysin

Target: Synaptophysin (SYP)

Alternative Name: SYP (SYP Products)

Background: Major synaptic vesicle protein p38, SYP,Synaptophysin is the major integral membrane protein of small synaptic vesicles1-3. It belongs to a family of proteins which includes synaptogyrin and synaptoporin. The protein contains 4 transmembrane domains and cytoplasmic N- and C-termini4. There are two intravesicular loops that contain disulfide bonds. The protein is N-glycosylated on the first intravesicular loop, a modification important for the synaptic vesicle targeting of synaptophysin. Indeed, a mutant form of the protein, unable to undergo glycolysation, leads to the accumulation of the protein in the cell body of neurons indicating that N-glycosylation is required for the synaptic localization of synaptophysin5. The C-terminal tail is extensively phosphorylated on Tyr residues. However, the biological significance of the post-translational modification is not yet determined6.Although exact and specific role of synaptophysin remains a mystery, a study suggests that the protein is required for efficient endocytosis of synaptic vesicles in cultured hippocampal neurons6.Since synaptophysin is exclusively detected in synaptic vesicles, it is widely used as a marker for presynaptic vesicles.Genetic screening in humans and behavioral studies in rodents show that loss of synaptophysin expression or expression of a truncated form of the protein may be attributed to mental retardation and/or learning deficits7,8. Interestingly though, synaptic transmission in synaptophysin knockout mice remains normal6.

Alternative names: Major synaptic vesicle protein p38, SYP

Gene ID: 24804

NCBI Accession: NM_003179

UniProt: P07825

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity