TRPC3 antibody (Intracellular)

Catalog No. ABIN7043818

Product Details anti-TRPC3 Antibody

Target: TRPC3 (Transient Receptor Potential Cation Channel, Subfamily C, Member 3 (TRPC3))

Binding Specificity: AA 822-835, Intracellular

Reactivity: Mouse

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This TRPC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Guinea Pig Polyclonal Antibody to TRPC3 Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,rat - 13,14 amino acid residues identical

Characteristics: Guinea pig Anti-TRPC3 Antibody is directed against an epitope of the mouse TRPC3 channel. Guinea pig Anti-TRPC3 Antibody (#) raised in guinea pig can be used in western blot analysis. It has been designed to recognize TRPC3 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-TRPC3 Antibody (ABIN7043820, ABIN7043966 and ABIN7043967)) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: HKLSEKLNPSVLRC, corresponding to amino acid residues 822-835 of mouse TRPC3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: BLP-CC016

Blocking Peptide: BLP-CC016

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TRPC3

Target: TRPC3 (Transient Receptor Potential Cation Channel, Subfamily C, Member 3 (TRPC3))

Alternative Name: TRPC3 (TRPC3 Products)

Background: Short transient receptor potential channel 3, Transient receptor protein 3, TRP3,The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals, about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin),TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities: 1. TRPC1 2. TRPC4 and TRPC5 3. TRPC3, TRPC6, TRPC7 4. TRPC2.2,5 They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.TRPC3, TRPC6 and TRPC7 form non-selective cationic channels that are activated by the stimulation of GPCRs.Different modes of activation, DAG and SOC, were described for the TRPC3 channel by different investigators working with heterologous expression systems.6,7

Alternative names: TRPC3, Short transient receptor potential channel 3, Transient receptor protein 3, TRP3

Gene ID: 22065

NCBI Accession: NM_003305

UniProt: Q9QZC1