TRPC3 antibody (Intracellular) (Atto 594)

Catalog No. ABIN7043819

The Rabbit Polyclonal anti-TRPC3 antibody is suitable to detect TRPC3 in samples from Mouse. It has been validated for IHC and IF.

Quick Overview for TRPC3 antibody (Intracellular) (Atto 594) (ABIN7043819)

Target: TRPC3 (Transient Receptor Potential Cation Channel, Subfamily C, Member 3 (TRPC3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPC3 antibody is conjugated to Atto 594

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: KO Validated

Product Details anti-TRPC3 Antibody (Atto 594)

Binding Specificity: AA 822-835, Intracellular

Purpose: A Rabbit Polyclonal Antibody to TRPC3 Channel Conjugated to the Fluorescent Dye ATTO-594

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,rat - 13,14 amino acid residues identical

Characteristics: Anti-TRPC3 Antibody (ABIN7043820, ABIN7043966 and ABIN7043967) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize TRPC3 from mouse, rat, and human samples. \nAnti-TRPC3-ATTO Fluor-594 Antibody (ABIN7043819) is directly labeled with fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-TRPC3-ATTO Fluor-594 Antibody can be used in immunohistochemistry applications and is specially suited to experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: HKLSEKLNPSVLRC, corresponding to amino acid residues 822-835 of mouse TRPC3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:50-1:60

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7237417)

Blocking Peptide: (ABIN7237417)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TRPC3

Target: TRPC3 (Transient Receptor Potential Cation Channel, Subfamily C, Member 3 (TRPC3))

Alternative Name: TRPC3

Background: Short transient receptor potential channel 3, Transient receptor protein 3, TRP3,The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel family and consists of diverse groups of proteins. In mammals about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities: TRPC1, TRPC4 and TRPC5, TRPC3, TRPC6, TRPC7 and TRPC2.2,5 They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.TRPC3, TRPC6 and TRPC7 form non-selective cationic channels that are activated by the stimulation of GPCRs.TRPC3 was shown to be activated by DAG and SOC, in heterologous expression systems.6,7

Alternative names: TRPC3, Short transient receptor potential channel 3, Transient receptor protein 3, TRP3

Gene ID: 22065

NCBI Accession: NM_003305

UniProt: Q9QZC1