TRPV2 antibody (Extracellular)

Catalog No. ABIN7043845

Product Details anti-TRPV2 Antibody

Target: TRPV2 (Transient Receptor Potential Cation Channel, Subfamily V, Member 2 (TRPV2))

Binding Specificity: AA 413-428, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPV2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Live Cell Imaging (LCI), Immunofluorescence (Cultured Cells) (IF (cc)), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to TRPV2 (VRL1) Channel

Specificity: 1st extracellular loop

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - 15,16 amino acid residues identicalThe antibody will not recognize human TRPV2

Characteristics: Anti-TRPV2 (VRL1) (extracellular) Antibody (ABIN7043845, ABIN7044002 and ABIN7044003)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, live cell imaging, and indirect flow cytometry applications. It has been designed to recognize TRPV2 from mouse and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HQPSLDQPAIPSSKAT, corresponding to amino acid residues 413-428 of rat TRPV2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-CC039

Blocking Peptide: BLP-CC039

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TRPV2

Target: TRPV2 (Transient Receptor Potential Cation Channel, Subfamily V, Member 2 (TRPV2))

Alternative Name: TRPV2 (TRPV2 Products)

Background: Transient receptor potential cation channel subfamily V member 2, Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2, Stretch-activated channel 2B, SAC2B,TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have six putative transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-termini of all TRPs are intracellular3.According to IUPHAR, the TRP family is comprised of numerous subfamilies on the basis of sequence homology, TRPC, TRPM, TRPV, TRPA, TRPML, and TRPP1-4. The TRPV subfamily consists of six members, TRPV1-65.Four members of the TRPV family have been described as thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (52 °C) 6,7. Although it shares around 50 % homology with TRPV1, TRPV2 is not activated by capsaicin nor by protons. It has a high temperature threshold of ~52 °C and is considered to play an essential role in the perception of high-intensity noxious heat stimulation8-10. TRPV2 is also considered to be a stretch-activated channel and to play a role in skeletal and cardiac muscle degeneration and pain pathway8. The TRPV2 channel is expressed in DRG neurons, different brain regions and non-neuronal tissues such as the spleen, lung and intestine and in components of the immune system5,11,12.

Alternative names: TRPV2 (VRL1), Transient receptor potential cation channel subfamily V member 2, Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2, Stretch-activated channel 2B, SAC2B

Gene ID: 29465

NCBI Accession: NM_016113

UniProt: Q9WUD2

Pathways: Regulation of Cell Size