TRPV6 antibody (Intracellular)

Catalog No. ABIN7043857

Product Details anti-TRPV6 Antibody

Target: TRPV6 (Transient Receptor Potential Cation Channel, Subfamily V, Member 6 (TRPV6))

Binding Specificity: AA 712-725, Intracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPV6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to TRPV6 Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse - 13,14 amino acid residues identical

Characteristics: Anti-TRPV6 Antibody is directed against an intracellular epitope of the human TRPV6. Anti-TRPV6 Antibody (ABIN7043857, ABIN7043998 and ABIN7043999) can be used in western blot, immunoprecipitation, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize TRPV6 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)NRGLEDGESWEYQI, corresponding to amino acid residues 712-725 of human TRPV6

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC|IFC

Negative Control: (ABIN7237506)

Blocking Peptide: (ABIN7237506)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TRPV6

Target: TRPV6 (Transient Receptor Potential Cation Channel, Subfamily V, Member 6 (TRPV6))

Alternative Name: TRPV6 (TRPV6 Products)

Background: Epithelial calcium channel 2, ECaC2, Calcium transport protein 1, CaT1, CaT-like, CaT-L, Transient receptor potential cation channel subfamily V member 6,TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology, TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family, the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5TRPV6 (CaT1) and TRPV5 (ECaC) are unique members of the TRP family. Both are epithelial calcium channels, form constitutively open channels, share high degree of homology (about 66 % ) with differences in the N and C-terminus. TRPV6 and TRPV5 are expressed in a 1,25-dihydroxyvitamin D3 dependent manner. In contrast to other members of the TRP family, TRPV6 and TRPV5 are highly Ca2+ selective and are expressed in Ca2+ transporting epithelia where they are assumed to have an important role in Ca2+ reabsorption. TRPV6 is highly expressed in the placenta, small intestine and kidney.6,7While not being expressed in benign prostate tissue, TRPV6 was found to be upregulated in prostate cancer tissue and correlation between expression and tumor grade was shown. Finally, TRPV6 was suggested as prognostic marker and as a promising candidate for therapeutic strategies.8,9

Alternative names: TRPV6, Epithelial calcium channel 2, ECaC2, Calcium transport protein 1, CaT1, CaT-like, CaT-L, Transient receptor potential cation channel subfamily V member 6

Gene ID: 55503

NCBI Accession: NM_014274

UniProt: Q9H1D0

Pathways: TCR Signaling