UCP2 antibody (Intracellular)

Catalog No. ABIN7043873

Product Details anti-UCP2 Antibody

Target: UCP2 (Uncoupling Protein 2 (Mitochondrial, Proton Carrier) (UCP2))

Binding Specificity: AA 153-164, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Mitochondrial Uncoupling Protein 2

Specificity: Intracellular

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 11,12 amino acid residues identical

Characteristics: Anti-UCP2 Antibody is directed against an epitope of the rat mitochondrial uncoupling protein 2 (UCP2). Anti-UCP2 Antibody (ABIN7043330, ABIN7044787 and ABIN7044788)) can be used in western blot and immunocytochemistry applications. It has been designed to recognize UCP2 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GRRYQSTVEAYK, corresponding to amino acid residues 153-164 of rat mitochondrial uncoupling protein 2 (UCP2)

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-NT128

Blocking Peptide: BLP-NT128

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for UCP2

Target: UCP2 (Uncoupling Protein 2 (Mitochondrial, Proton Carrier) (UCP2))

Alternative Name: UCP2 (UCP2 Products)

Background: Mitochondrial uncoupling protein 2, UCPH, Solute carrier family 25 member 8, SLC25A8, BMIQ4,Mitochondrial carriers (MCs) transport a variety of metabolites across the inner mitochondrial membrane. All MCs belong to the same family since their polypeptide chains consist of three tandemly related sequences of about 100 amino acids. MCs are encoded in humans by SLC25 genes.UCP2 (Uncoupling protein 2) is a member of the MC family encoded by the SLC25A8 gene. It is expressed on the inner mitochondrial membrane of cells in the lungs, kidneys, spleen, heart and mainly in the hypothalamus, pituitary and brain stem. The expression of UCP2 is upregulated by fatty acids, prostaglandins, TNFα and other cytokines. UCP2 is involved in regulation of body temperature and the control of oxygen radical production.Each monomer of UCP2 has six helices crossing the cell membrane connected by hydrophilic loops with both the N- and C- termini on the cytosolic side of the inner mitochondrial membrane. UCP2 catalyzes the unidirectional transport of H+. Charge balance can be achieved by cotransport (symport) of solutes, counter-transport of solutes and uniport of electroneutral metabolites. The transport of H+ is stimulated by fatty acids and inhibited by purine nucleotides. In addition, activation of H+ transport by superoxidase has been observed.Recent studies suggest that UCP2 and other uncoupling proteins are involved in pathologies such as obesity, type-2 diabetes and atherosclerosis. Mice lacking UCP2 have been shown to over-produce reactive oxygen but not to gain weight or be insensitive to cold stimuli1.

Alternative names: UCP2, Mitochondrial uncoupling protein 2, UCPH, Solute carrier family 25 member 8, SLC25A8, BMIQ4

Gene ID: 54315

NCBI Accession: NM_003355

UniProt: P56500

Pathways: Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Proton Transport