UCP2 antibody (Internal Region)

Catalog No. ABIN5539644

Product Details anti-UCP2 Antibody

Target: UCP2 (Uncoupling Protein 2 (Mitochondrial, Proton Carrier) (UCP2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This UCP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: Uncoupling protein 2 / UCP2

Sequence: DSVKQFYTKG SEH

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Weak positive staining of red and white pulp cells in parafin embedded Human Kidney, at 6-7 μg/mL.

Western Blot: Approx 37 kDa band observed in Rat Adipose and 28-30 kDa in Mouse Spleen lysates (calculated MW of 33.4 kDa according to Mouse NP_035801.3 and 33.4 kDa according to Rat NP_062227.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 h

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the Mitochondria of MCF7 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for UCP2

Target: UCP2 (Uncoupling Protein 2 (Mitochondrial, Proton Carrier) (UCP2))

Alternative Name: UCP2 (UCP2 Products)

Synonyms: UCP2 antibody, DKFZp470P1633 antibody, ucp2 antibody, MGC53319 antibody, MGC75881 antibody, cb74 antibody, ATUCP2 antibody, K19M22.21 antibody, K19M22_21 antibody, uncoupling protein 2 antibody, BMIQ4 antibody, SLC25A8 antibody, UCPH antibody, Slc25a8 antibody, mitochondrial uncoupling protein 2 antibody, uncoupling protein 2 antibody, uncoupling protein 2 (mitochondrial, proton carrier) L homeolog antibody, uncoupling protein 2 (mitochondrial, proton carrier) antibody, uncoupling protein 1 antibody, PTRG_09289 antibody, UCP2 antibody, LOC100282746 antibody, ucp2.L antibody, ucp2 antibody, ucp1 antibody, Ucp2 antibody

Background: UCP2, uncoupling protein 2 (mitochondrial, proton carrier), SLC25A8, UCPH , uncoupling protein 2, uncoupling protein-2

Gene ID: 7351, 22228, 54315

NCBI Accession: NP_003346

Pathways: Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Proton Transport