Recombinant FCGR1A antibody

Catalog No. ABIN7072384

Product Details anti-FCGR1A Antibody

Target: FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This FCGR1A antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Cytotoxicity Test (CyTox)

Purpose: Anti-CD64 [H22], Rabbit IgG, kappa

Specificity: This antibody is specific for human CD64, specifically an epitope outside the Fc binding domain. The binding activity of the VH/VL antibody format was unaffected by the fusion of the m22(scFv) coding regions to the truncated ETA coding sequences.

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This recombinant antibody was prepared by cloning the single chain fragment (scFv) derived from the murine anti-human CD64 mAb m22 into the bacterial expression vector pBM1.1, and fusing it to a deletion mutant of Pseudomonas exotoxin A (ETA' ).

Clone: H22

Isotype: IgG kappa

Application Details

Application Notes: This antibody binds human CD64 and can be bound to a toxin so that together it displays specific cytotoxicity, and thus can be used to selectively eliminate AML cells, FC analysis indicated that this immunotoxin drove 41 % of primary leukemia cells from a patient with CD64-positive AML into early apoptosis (Tur, 2003). Similarly, in an in vivo mouse model, IHC analysis revealed efficient elimination of human CD64+ tumor cells in mouse organs (Tur, 2011).

Comment:

This is a chimeric rabbit IgG antibody based on the original human IgG1 antibody, created for improved compatibility with existing reagents, assays, and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for FCGR1A

Target: FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))

Alternative Name: CD64 (FCGR1A Products)

Background: FcγRI, High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc-gamma RI, FcRI, Fc-gamma RIA

UniProt: P12314

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process