The mouse monoclonal antibody 10.1 recognizes an extracellular epitope on CD64/FcgammaRI, a 72 kDa single chain type I glycoprotein, that is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The epitope is sensitive to formalin fixation.
Cross-Reactivity (Details)
Human, Non-Human Primates
Purification
Purified by protein-A affinity chromatography.
Purity
> 95 % (by SDS-PAGE)
Endotoxin Level
Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Immunogen
Rheumatoid synovial fluid cells and fibronectin purified human monocytes
Functional application: Blocking of IgG binding to the FcγR1. Flow cytometry: Recommended dilution: 1-4 μg/mL. Immunohistochemistry (paraffin sections and frozen sections): There can occur problems with paraformaldehyde fixation.
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.4
Preservative
Azide free
Storage
4 °C
Storage Comment
Store at 2-8°C. Do not freeze.
Devaraj, Davis, Simon, Jialal: "CRP promotes monocyte-endothelial cell adhesion via Fcgamma receptors in human aortic endothelial cells under static and shear flow conditions." in: American journal of physiology. Heart and circulatory physiology, Vol. 291, Issue 3, pp. H1170-6, (2006) (PubMed).
Devaraj, Du Clos, Jialal: "Binding and internalization of C-reactive protein by Fcgamma receptors on human aortic endothelial cells mediates biological effects." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 25, Issue 7, pp. 1359-63, (2005) (PubMed).
Beekman, Bakema, van der Linden, Tops, Hinten, van Vugt, van de Winkel, Leusen: "Modulation of FcgammaRI (CD64) ligand binding by blocking peptides of periplakin." in: The Journal of biological chemistry, Vol. 279, Issue 32, pp. 33875-81, (2004) (PubMed).
Sánchez-Torres, García-Romo, Cornejo-Cortés, Rivas-Carvalho, Sánchez-Schmitz: "CD16+ and CD16- human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells." in: International immunology, Vol. 13, Issue 12, pp. 1571-81, (2001) (PubMed).
Brichard, Varis, Latinne, Deneys, de Bruyere, Leveugle, Cornu: "Intracellular cytokine profile of cord and adult blood monocytes." in: Bone marrow transplantation, Vol. 27, Issue 10, pp. 1081-6, (2001) (PubMed).
Fadlon, Vordermeier, Pearson, Mire-Sluis, Dumonde, Phillips, Fishlock, Brown: "Blood polymorphonuclear leukocytes from the majority of sickle cell patients in the crisis phase of the disease show enhanced adhesion to vascular endothelium and increased expression of CD64." in: Blood, Vol. 91, Issue 1, pp. 266-74, (1998) (PubMed).
Hashimoto, Yamada, Motoyoshi, Akagawa: "Enhancement of macrophage colony-stimulating factor-induced growth and differentiation of human monocytes by interleukin-10." in: Blood, Vol. 89, Issue 1, pp. 315-21, (1997) (PubMed).
Jayaram, Buckle, Hogg: "The Fc receptor, FcRI, and other activation molecules on human mononuclear phagocytes after treatment with interferon-gamma." in: Clinical and experimental immunology, Vol. 75, Issue 3, pp. 414-20, (1989) (PubMed).
Dougherty, Selvendran, Murdoch, Palmer, Hogg: "The human mononuclear phagocyte high-affinity Fc receptor, FcRI, defined by a monoclonal antibody, 10.1." in: European journal of immunology, Vol. 17, Issue 10, pp. 1453-9, (1987) (PubMed).
Target
FCGR1A
(Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))
CD64 antibody, CD64A antibody, FCRI antibody, IGFR1 antibody, FcgammaRI antibody, Fcgr1 antibody, Fcgr1b antibody, AI323638 antibody, AV092959 antibody, IGGHAFC antibody, FCGR1A antibody, Fc fragment of IgG receptor Ia antibody, Fc receptor, IgG, high affinity I antibody, FCGR1A antibody, Fcgr1a antibody, Fcgr1 antibody
Background
Fc fragment of IgG receptor Ia,CD64 (FcgammaRI) is a cell surface receptor for Fc region of IgG. It is composed of specific ligand binding alpha subunit and promiscuous gamma subunit, which is indispensable for tyrosine-based signaling. However, even the alpha subunit can transduce signals leading to cellular effector functions. The isoform FcgammaRIa1 binds human IgG with high affinity, has limited myeloid cell distribution, and a relatively large intracellular domain. Products of related genes include FcgammaRIb and FcgammaRIc isoforms, but these specify low affinity IgG receptors if functionally expressed at all. Besides a role in antigen clearance, FcgammaRI (a1) can potently enhance MHC class I and II antigen presentation in vitro and in vivo.,FcRI, IGFR1, FcgR1A