Recombinant IL-6 Receptor antibody

Catalog No. ABIN7072548

Product Details anti-IL-6 Receptor Antibody

Target: IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This IL-6 Receptor antibody is un-conjugated

Application: Flow Cytometry (FACS), Blocking Peptide (BP)

Purpose: Anti-IL-6R [D7715A7], Rabbit IgG, kappa

Specificity: This antibody is specific for murine IL-6R.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising Lewis rats with OKT4 cells.

Clone: D7715A7

Isotype: IgG kappa

Application Details

Application Notes: This antibody binds to IL-6R-positive cells, and blocks IL-6 binding (Vink et al, 1990, Coulie et al, 1990). This antibody is able to inhibit the growth of IL6-dependent plasmacytoma T1033C2 in vitro (Coulie et al, 1990). Administration of this antibody to mice prior to challenge with T1033C2 resulted in a protection rate of 60 % and prolonged survival in tumour-bearing mice (Vink et al, 1990). This antibody has been used in flow cytometry to assess IL-6Ra expression (Hoge et al, 2013). When added to dendritic cells subject to CD40 activation in vitro, this antibody neutralises IL-6 and ablates the effect of CD40 activation (Grohmann et al, 2001).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for IL-6 Receptor

Target: IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))

Alternative Name: IL-6R (IL6R Products)

Background: CD126, 15A7, IL-6Ra, IL6R, Interleukin-6 receptor subunit alpha, Il6ra, IL-6 receptor subunit alpha, IL-6R subunit alpha, IL-6R-alpha, IL-6RA, IL-6R 1

UniProt: P22272

Pathways: JAK-STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints