AP2A2 antibody (AA 590-939)

Catalog No. ABIN7144362

Product Details anti-AP2A2 Antibody

Target: AP2A2 (Adaptor-Related Protein Complex 2, alpha 2 Subunit (AP2A2))

Binding Specificity: AA 590-939

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AP2A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Cross-Reactivity: Human, Mouse

Purification: Antigen Affinity Purified

Immunogen: Recombinant Human AP-2 complex subunit alpha-2 protein (590-939AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for AP2A2

Target: AP2A2 (Adaptor-Related Protein Complex 2, alpha 2 Subunit (AP2A2))

Alternative Name: AP2A2 (AP2A2 Products)

Background:

Background: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity).

Aliases: 100 kDa coated vesicle protein C antibody, Adaptor protein complex AP-2 subunit alpha-2 antibody, Adaptor-related protein complex 2 subunit alpha-2 antibody, Alpha-adaptin C antibody, Alpha2-adaptin antibody, AP-2 complex subunit alpha-2 antibody, Ap2a2 antibody, AP2A2_HUMAN antibody, Clathrin assembly protein complex 2 alpha-C large chain antibody, HIP-9 antibody, Huntingtin yeast partner J antibody, Huntingtin-interacting protein 9 antibody, Huntingtin-interacting protein J antibody, Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit antibody

UniProt: O94973

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation, SARS-CoV-2 Protein Interactome