SF3B4 antibody (AA 374-424)

Catalog No. ABIN7454988

Product Details anti-SF3B4 Antibody

Target: SF3B4 (Splicing Factor 3b, Subunit 4, 49kDa (SF3B4))

Binding Specificity: AA 374-424

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SF3B4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purpose: Rabbit anti-SF3B4 Antibody, Affinity Purified

Predicted Reactivity: Rat

Purification: Affinity Purified

Immunogen: Between AA 374 and 424

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for SF3B4

Target: SF3B4 (Splicing Factor 3b, Subunit 4, 49kDa (SF3B4))

Alternative Name: SF3B4 (SF3B4 Products)

Background: Background: Splicing factor 3b, subunit 4, 49 kDa (SF3B4) is one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B [taken from NCBI Entrez Gene (Gene ID: 10262)].

Gene ID: 10262

NCBI Accession: NP_005841

UniProt: Q15427