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Leptin antibody (AA 101-154)

Name: Leptin antibody (AA 101-154)
SKU: ABIN725030
Price: 384.62 USD
Availability: InStock

LEP Reactivity: Mouse, Rat ELISA, FACS, IF (cc), IF (p), IHC (fro), IHC (p) Host: Rabbit Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Leptin Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.

IHC (p)

Formalin-fixed and paraffin embedded: rat liver labeled with Anti-Leptin Polyclonal Antibody (ABIN725030), Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining

IHC

Paraformaldehyde-fixed, paraffin embedded mouse liver, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Leptin Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.

IHC (p)

3 images

Catalog No. ABIN725030

$384.62

Plus shipping costs $45.00

100 μL

Shipping to: United States

Delivery in 4 to 6 Business Days

Target See all Leptin (LEP) Antibodies

Leptin (LEP)
Binding Specificity
26

15

15

15

15

13

10

7

5

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
AA 101-154
Reactivity
135

74

70

12

8

7

5

3

3

3

2

2

2

1

1
Mouse, Rat
Host
176

28

7

6

3
Rabbit
Compatible Secondaries
Clonality
189

29
Polyclonal
Conjugate
112

28

17

9

8

7

3

3

3

3

3

3

3

3

3

3

3

3

3
This Leptin antibody is un-conjugated
Application
129

90

69

39

38

38

37

31

24

18

15

11

10

9

6

4

2

2

1

1

1

1

1

1

1
ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity

Dog,Cow,Pig,Horse

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from mouse Leptin

Isotype

IgG
anti-Leptin (LEP) (AA 8-37) antibody
LEP Reactivity: Human FACS, IF, IHC, ELISA, WB Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 17-100) antibody
LEP Reactivity: Human, Mouse, Rat ELISA, IF (cc), IF (p), IHC (fro), IHC (p), WB Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 7-37), (N-Term) antibody
LEP Reactivity: Human FACS, IF, IHC, WB Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 7-37), (N-Term) antibody
LEP Reactivity: Human EIA, FACS, IF, IHC (p), WB Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) antibody
LEP Reactivity: Human, Mouse, Rat IHC Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP Reactivity: Human IF, WB Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (N-Term) antibody
LEP Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Sheep IHC, WB Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 22-167) antibody
LEP Reactivity: Mouse ICC, IHC, IP, WB Host: Rabbit Monoclonal C1 unconjugated

anti-Leptin (LEP) (Middle Region) antibody
LEP Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Horse, Pig, Rabbit, Sheep IHC, WB Host: Rabbit Polyclonal unconjugated

anti-Leptin (LEP) (AA 134-166) antibody
LEP Reactivity: Human IF, ELISA, WB Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

Leptin (LEP)

Alternative Name

Leptin (LEP Products)

Synonyms

ob antibody, obese antibody, LEPD antibody, OB antibody, OBS antibody, leptin antibody, Lep antibody, LEP antibody, lep antibody

Background

May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
Subcellular location: Secreted
Synonyms: ob, obese, Leptin, Obesity factor, Lep

Gene ID

16846

UniProt

P41160

Pathways

JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process