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NR1I2 antibody (AA 101-150)

Name: NR1I2 antibody (AA 101-150)
SKU: ABIN738816
Price: 384.62 USD
Availability: InStock

NR1I2 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-PAR-2 Polyclonal Antibody, Unconjugated (ABIN738816) at 1:200 followed by conjugation to the secondary antibody and DAB staining (NR1I2 antibody (AA 101-150))

IHC

ABIN738816 PAR-2 IHC human colon (NR1I2 antibody (AA 101-150))

IHC

Lane 1: mouse brain lysates Lane 2: mouse embryo lysates probed with Anti PAR-2 Polyclonal Antibody, Unconjugated (ABIN738816) at 1:200 in 4 °C. (NR1I2 antibody (AA 101-150))

SDS

Formalin-fixed and paraffin embedded rat colitis tissue labeled with Anti-PAR-2 Polyclonal Antibody, Unconjugated (ABIN738816) at 1:200 followed by conjugation to the secondary antibody and DAB staining (NR1I2 antibody (AA 101-150))

IHC

Formalin-fixed and paraffin embedded rat colitis tissue labeled with Anti-PAR-2 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining (NR1I2 antibody (AA 101-150))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded human colon cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-PAR-2 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining. (NR1I2 antibody (AA 101-150))

IHC (p)

6 images

Catalog No. ABIN738816

Datasheet as PDF

Target See all NR1I2 Antibodies

NR1I2 (Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2))
Binding Specificity
16

14

7

6

4

4

3

3

3

3

2

1

1

1

1

1

1

1

1

1

1

1
AA 101-150
Reactivity
73

46

31

18

2

2

2

2

1

1

1
Human, Mouse, Rat
Host
78

7

1
Rabbit
Compatible Secondaries
Clonality
81

5
Polyclonal
Conjugate
36

8

7

4

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This NR1I2 antibody is un-conjugated
Application
72

25

18

16

12

12

12

6

6

4

4

2

2

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Human, Mouse, Rat

Predicted Reactivity

Rabbit

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human PAR2

Isotype

IgG
anti-Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2) (AA 100-127) antibody
NR1I2 Reactivity: Human WB, FACS, IHC (p), IF Host: Rabbit Polyclonal RB21890 unconjugated

anti-Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2) (AA 1-142) antibody
NR1I2 Reactivity: Human ELISA, FACS, ICC Host: Mouse Monoclonal 1D12G1 unconjugated

anti-Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2) (C-Term) antibody
NR1I2 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2) (AA 1-142) antibody
NR1I2 Reactivity: Human ELISA, FACS Host: Mouse Monoclonal 6H11D8 unconjugated

anti-Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2) (AA 51-150) antibody
NR1I2 Reactivity: Human, Mouse, Pig WB, ELISA, FACS, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2) (AA 1-434) antibody
NR1I2 Reactivity: Human WB, IF Host: Mouse Polyclonal unconjugated

anti-Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2) (Internal Region) antibody
Verified NR1I2 Reactivity: Human WB, ELISA, IHC Host: Goat Polyclonal unconjugated

anti-Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2) (AA 279-378) antibody
NR1I2 Reactivity: Human WB, ELISA Host: Mouse Polyclonal unconjugated

anti-Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2) (AA 108-157) antibody
NR1I2 Reactivity: Human WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2) (AA 1-434) antibody
NR1I2 Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

NR1I2 (Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2))

Alternative Name

PAR-2 (NR1I2 Products)

Background

Synonyms: PAR2, GPR11, Proteinase-activated receptor 2, PAR-2, Coagulation factor II receptor-like 1, G-protein coupled receptor 11, Thrombin receptor-like 1, F2RL1
Background: Coagulation factor II (thrombin) receptor-like 1 (F2RL1) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL1 is also a member of the protease-activated receptor family. It is activated by trypsin, but not by thrombin. It is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. The F2RL1 gene contains two exons and is widely expressed in human tissues. The predicted protein sequence is 83 % identical to the mouse receptor sequence. [provided by RefSeq, Jul 2008]

Gene ID

2150

UniProt

P55085

Pathways

Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway