Nav1.8 antibody (AA 1724-1956)

Catalog No. ABIN863135

Product Details anti-Nav1.8 Antibody

Target: Nav1.8 (SCN10A) (Sodium Channel, Voltage-Gated, Type X, alpha Subunit (SCN10A))

Binding Specificity: AA 1724-1956

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nav1.8 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Antibody Array (AA)

Specificity: Detects ~220 kDa. No cross reactivity against other Nav channels.

Cross-Reactivity: Human, Monkey, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 1724-1956 of rat Nav1.8

Clone: S134

Isotype: IgG2a

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN863135 was sufficient for detection of Nav1.8 in 10 μg of COS cell lysate transiently expressing Nav1.8 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References for anti-Nav1.8 antibody (ABIN863135)

Ngwainmbi, De, Smith, El-Hage, Fitting, Kang, Dewey, Hauser, Akbarali: "Effects of HIV-1 Tat on enteric neuropathogenesis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 43, pp. 14243-51, (2014) (PubMed).

Target Details for Nav1.8

Target: Nav1.8 (SCN10A) (Sodium Channel, Voltage-Gated, Type X, alpha Subunit (SCN10A))

Alternative Name: Nav1.8 (SCN10A Products)

Background: Nav1.8 is a voltage-gated sodium channel and plays a critical role in the generation and conduction of action potentials and is thus important for electrical signaling by most excitable cells. Therapeutically, the association of pain insensitivity with the loss of function of a certain sodium channel may have implications. Since Nav1.8 is not present in cardiac muscle or neurons in the central nervous system, blockers of Nav1.8 will not have direct action on these cells and thus can have less side effects than current pain medications. By performing more studies, there is a possibility to develop a new generation of drugs that can reduce the pain intensity in animals.

Gene ID: 29571

NCBI Accession: NP_058943

UniProt: Q62968