ATP1B1 antibody (ATPase, Na+/K+ Transporting, beta 1 Polypeptide) (Subunit beta)

Details for Product anti-ATP1B1 Antibody No. ABIN93533
Antigen
Epitope
Subunit beta
14
9
7
7
4
4
4
2
2
1
1
1
1
1
1
Reactivity
Spinach (Spinacia oleracea), Synechocystis PCC 6803
79
46
42
9
6
6
5
5
4
4
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Host
Chicken
82
7
4
Clonality
Polyclonal
Conjugate
This ATP1B1 antibody is un-conjugated
7
5
5
3
3
3
2
2
2
2
2
2
Application
Immunolocalization (IL), Western Blotting (WB)
76
47
44
8
3
3
3
3
2
2
1
1
1
Options
Immunogen KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta UniProt: P19366, TAIR: AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1, UniProt: P83483, TAIR: At5g08670 as well as Chlamydomonas reinhardtii, UniProt: P06541 and A8IQU3
Isotype IgY
Cross-Reactivity (Details) No cross-reactivity with: archeal V-type ATP synthase
Characteristics Expected / apparent Molecular Weight of the Antigene: 53.9kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)
Purification affinity purified
Antigen
Alternative Name AtpB (ATP1B1 Antibody Abstract)
Background AGI Code: At5g08670, ATCG00480
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.
Molecular Weight 53.9 kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)
UniProt P83483, A8IQU3, P19366, P06541
Pathways Thyroid Hormone Synthesis, Ribonucleoside Biosynthetic Process, SARS-CoV-2 Protein Interactome
Application Notes Recommended Dilution: 1 : 5 000 - 1 : 8 000 (WB), 1 : 500 for localization of native enzyme by immunogold(IL).
Comment

results of immunogold studies using anti-AtpB antibody are published in Andersson et al. (2009)

Restrictions For Research Use only
Format Liquid
Buffer PBS pH 8.0 + 0.02 % sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Make aliquots to avoid repreated freeze-thaw cycles and working with a stock.
Storage 4 °C
Supplier Images
Image no. 1 for anti-ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1) (Subunit beta) antibody (ABIN93533) From left to right: molecular weight markers (MagicMark XP, Invitrogen), whole cell e...
Product cited in: Fuller, Craven, Hall, Kijek, Taft-Benz, Kawula: "RipA, a cytoplasmic membrane protein conserved among Francisella species, is required for intracellular survival." in: Infection and immunity, Vol. 76, Issue 11, pp. 4934-43, 2008 (PubMed).