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CD11b antibody (Integrin alpha M) (FITC) Primary Antibody

ITGAM Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-174 FITC
Pubmed (2)
Catalog No. ABIN93959
$455.40
Plus shipping costs $45.00
100 tests
local_shipping Shipping to: United States
Delivery in 5 to 7 Business Days
  • Target
    CD11b (ITGAM)
    Reactivity
    • 308
    • 208
    • 63
    • 48
    • 29
    • 28
    • 20
    • 17
    • 14
    • 10
    • 9
    • 7
    • 7
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    Human
    Host
    • 189
    • 147
    • 104
    • 7
    • 5
    • 1
    Mouse
    Clonality
    • 342
    • 107
    Monoclonal
    Conjugate
    • 143
    • 43
    • 43
    • 34
    • 24
    • 9
    • 9
    • 9
    • 8
    • 7
    • 6
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD11b antibody is conjugated to FITC
    Application
    • 357
    • 111
    • 104
    • 86
    • 83
    • 59
    • 50
    • 35
    • 25
    • 21
    • 14
    • 14
    • 8
    • 8
    • 7
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP)
    Specificity
    The antibody MEM-174 recognizes CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.
    HLDA VI, WS Code BP 310
    HLDA VI, WS Code M 18
    Cross-Reactivity (Details)
    Human
    Immunogen
    Human granulocytes
    Clone
    MEM-174
    Isotype
    IgG2a
  • Application Notes
    The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
    Comment

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

    Restrictions
    For Research Use only
  • Reconstitution
    No reconstitution is necessary.
    Buffer
    The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Avoid prolonged exposure to light.
    Storage
    4 °C
    Storage Comment
    Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
  • Hasan, Osickova, Bumba, Novák, Sebo, Osicka: "Interaction of Bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding." in: FEBS letters, Vol. 589, Issue 3, pp. 374-9, 2015 (PubMed).

    Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

  • Target
    CD11b (ITGAM)
    Alternative Name
    CD11b (ITGAM Antibody Abstract)
    Synonyms
    CD11b/CD18, CR3, CR3A, Cd11b, F730045J24Rik, Ly-40, MAC1, Mac-1, Mac-1a, CD11B, MAC-1, MAC1A, MO1A, SLEB6, C3bi, integrin subunit alpha M, integrin alpha M, integrin, alpha M (complement component 3 receptor 3 subunit), integrin alpha-M, ITGAM, Itgam, LOC100351865
    Background
    CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
    Pathways
    Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
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