CD2 antibody (CD2 Molecule) (Biotin)

Details for Product anti-CD2 Antibody No. ABIN94022
Antigen
Reactivity
Human
480
100
68
32
24
21
15
13
13
12
11
7
7
6
5
5
4
3
2
2
2
2
1
1
Host
Mouse
448
88
53
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD2 antibody is conjugated to Biotin
65
60
48
22
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
414
134
105
85
85
63
49
42
32
13
9
9
4
4
2
1
1
1
Options
Immunogen Human peripheral T cells.
Clone MEM-65
Isotype IgG1
Specificity The antibody MEM-65 recognizes an unique epitope of CD2, a 50 kDa glycoprotein present on the human peripheral blood T-lymphocytes and NK cells, also expressed by all thymocytes.
HLDA VI, WS Code T 6T-012
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD2 (CD2 Antibody Abstract)
Background CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.
Application Notes Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry.Suggested working dilution is 1:1000. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Comment

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: Espagnolle, Depoil, Zaru, Demeur, Champagne, Guiraud, Valitutti: "CD2 and TCR synergize for the activation of phospholipase Cgamma1/calcium pathway at the immunological synapse." in: International immunology, Vol. 19, Issue 3, pp. 239-48, 2007 (PubMed).

Drbal, Hilgert, Cebecauer, Angelisová, Horejsí: "New monoclonal antibodies to human leucocyte surface molecule CD2." in: Folia biologica, Vol. 43, Issue 6, pp. 245-6, 1998 (PubMed).