Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2) antibody

Details for Product No. ABIN94032
Antigen
Reactivity
Human, Pig (Porcine), Cow (Bovine), Dog (Canine)
307
51
45
42
40
23
17
17
12
11
6
3
3
3
1
Host
Mouse
281
96
3
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
61
58
25
11
5
4
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
269
108
83
69
61
50
43
36
18
13
12
8
6
6
3
2
1
1
Options
Immunogen IM9 human B-lymphoblastoid cell line
Clone LT21
Isotype IgG1
Specificity The antibody LT21 reacts with CD21 (CR2), a 145 kDa transmembrane glycoprotein (complement C3d receptor - C3dR) expressed on B lymphocytes, follicular dendritic cells, some epithelial cells and a subsets of T lymphocytes. It is not expressed on immature B cells.
HLDA VI, WS Code B CD21.1
Cross-Reactivity (Details) Human, Porcine, Bovine, Canine (Dog)
Purification Purified by precipitation and chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD21 (CR2 Antibody Abstract)
Background CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2) antibody (ABIN94032) Double staining of human, canine and porcine B lymphocytes with anti-CD79a (HM57) and...
Product cited in: Faldyna, Samankova, Leva, Cerny, Oujezdska, Rehakova, Sinkora: "Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs." in: Veterinary immunology and immunopathology, Vol. 119, Issue 1-2, pp. 56-62, 2007 (PubMed).

Filatov, Krotov, Zgoda, Volkov: "Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry." in: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 21-33, 2007 (PubMed).