CD34 antibody (CD34 Molecule) (APC)

Details for Product anti-CD34 Antibody No. ABIN94081
Antigen
Reactivity
Human
657
202
175
34
34
32
29
29
12
8
5
3
2
2
1
Host
Mouse
456
239
68
4
3
Clonality (Clone)
Monoclonal ()
Conjugate
This CD34 antibody is conjugated to APC
74
72
49
26
8
8
7
6
6
6
6
6
6
6
6
6
6
6
6
6
5
5
5
5
5
4
3
3
3
3
3
3
2
2
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
536
362
210
210
146
103
79
72
56
46
39
28
19
18
13
8
6
4
2
2
1
1
1
1
1
Options
Immunogen Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.
Clone 4H11[APG]
Isotype IgG1
Specificity The antibody 4H11[APG] reacts with Class III epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. The antibody 4H11[APG] completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
HLDA VI, WS Code M MA58
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD34 (CD34 Antibody Abstract)
Background CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD34 Molecule (CD34) antibody (APC) (ABIN94081) Detection of CD34 in human chronic myeloid leukemia cell line MOLM-7 with anti-human ...
Image no. 2 for anti-CD34 Molecule (CD34) antibody (APC) (ABIN94081) Flow Cytometry analysis Surface staining of Kg-1a human acute myelogenous leukemia ce...
Product cited in: Elknerová, Lacinová, Soucek, Marinov, Stöckbauer: "Growth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines." in: Neoplasma, Vol. 54, Issue 4, pp. 311-20, 2007 (PubMed).