CD59 antibody

Catalog No. ABIN94204

This Mouse Monoclonal antibody specifically detects CD59 in FACS and Func. It exhibits reactivity toward Human and Pig and has been mentioned in 2+ publications.

Quick Overview for CD59 antibody (ABIN94204)

Target: CD59

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD59 antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func)

Clone: MEM-129

Product Details anti-CD59 Antibody

Purpose: Anti-Hu CD59 Purified Azide Free

Specificity: The antibody MEM-129 reacts with CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored extracellular glycoprotein expressed on all hematopoietic cells, it is widely present on cells in all tissus.

Cross-Reactivity (Details): Human, Porcine

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human peripheral blood lymphocytes

Isotype: IgM

Application Details

Application Notes: Functional application: The antibody MEM-129 activates T cells.
Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0

Preservative: Azide free

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD59 antibody (ABIN94204)

Stulnig, Berger, Sigmund, Stockinger, Horejsí, Waldhäusl: "Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol." in: The Journal of biological chemistry, Vol. 272, Issue 31, pp. 19242-7, (1997) (PubMed).

Olweus, Lund-Johansen, Terstappen: "CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells." in: Blood, Vol. 85, Issue 9, pp. 2402-13, (1995) (PubMed).

Target Details for CD59

Target: CD59

Alternative Name: CD59

Background: CD59 Molecule,CD59 (protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.,MACIF, MAC-IP, protectin, MIRL, HRF-20, HRF20, 1F5, MIN

Gene ID: 966

UniProt: P13987

Pathways: Complement System