CD8 antibody (Biotin)

Details for Product No. ABIN94233
Antigen
Reactivity
Human
566
141
32
27
22
22
22
17
17
16
15
14
11
11
11
10
7
6
6
5
1
1
1
1
Host
Mouse
673
122
68
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
142
105
69
44
15
10
9
8
8
7
6
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Microcytotoxicity Assay (MCA)
715
152
131
127
111
59
54
48
40
35
13
13
12
9
6
6
4
4
1
1
1
Options
Immunogen Crude thymus membrane fraction.
Clone MEM-31
Isotype IgG2a
Specificity The antibody MEM-31 recognizes a conformationally-dependent epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8 alpha/alpha homodimer or CD8 alpha/beta heterodimer (each monomer approx. 32-34 kDa).
The antibody does not react with formaldehyde-fixed cells, negative in Western Blotting application.

HLDA III, WS Code T 575
Cross-Reactivity (Details) Human
Antigen
Background The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as alpha/beta heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as alpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha-beta but not alpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Application Notes Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry.Suggested working dilution is 1:2500. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Comment

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD8 antibody (Biotin) (ABIN94233) Surface staining of human peripheral blood lymphocytes using anti-human CD8
Product cited in: Linnebacher, Wienck, Boeck, Klar: "Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO)." in: Journal of biomedicine & biotechnology, Vol. 2010, pp. 841451, 2010 (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Estefanía, Flores, Gómez-Lozano, Aguilar, López-Botet, Vilches: "Human KIR2DL5 is an inhibitory receptor expressed on the surface of NK and T lymphocyte subsets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 7, pp. 4402-10, 2007 (PubMed).

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).