FAS antibody (Fas (TNF Receptor Superfamily, Member 6))

Details for Product anti-FAS Antibody No. ABIN94248
Antigen
Reactivity
Human
604
101
90
4
3
2
1
1
1
Host
Mouse
353
319
7
4
4
3
Clonality (Clone)
Monoclonal ()
Conjugate
This FAS antibody is un-conjugated
67
56
42
26
15
8
8
8
7
7
7
7
4
4
4
4
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
303
284
198
149
79
67
66
59
52
38
19
16
10
8
7
6
4
4
3
3
2
2
1
1
1
1
Options
Immunogen HUT-78 human T cell lymphoma cell line
Clone LT95
Isotype IgG1
Specificity The antibody LT95 reacts with CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells.
It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD95 / Fas (FAS Antibody Abstract)
Background CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.
Pathways p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Fas (TNF Receptor Superfamily, Member 6) (FAS) antibody (ABIN94248) Flow cytometry analysis of 5-bromodeoxyuridin (BrdU) incorporation in CEM human acute...