LYN antibody (V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog) (AA 8-238)

Details for Product anti-LYN Antibody No. ABIN94427
Antigen
Epitope
AA 8-238
26
16
16
16
11
9
8
8
7
7
6
6
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
228
147
114
16
16
6
5
4
4
3
3
2
2
2
1
Host
Mouse
200
34
7
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
This LYN antibody is un-conjugated
12
9
7
6
5
5
4
4
4
4
4
4
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
202
107
97
33
32
16
14
13
11
5
4
3
1
1
Options
Immunogen Bacterially expressed recombinant fragment of human Lyn (aa 8 - 238).
Clone LYN-01
Isotype IgG1
Specificity The antibody LYN-01 reacts with Lyn (p56/p53), a non-receptor Src-family tyrosine kinase expressed in hematopoietic tissues.
Cross-Reactivity (Details) Mouse, Human, Rat
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name Lyn (LYN Antibody Abstract)
Background Lyn is a Src-family protein tyrosine kinase that is predominantly expressed in hematopoietic cells. It is associated with a number of cell surface receptors including the B cell antigen receptor (BCR) and Fc receptors. Upon their triggering, Lyn phosphorylates subunits of these receptors in a cholesterol-dependent manner, utilizing the plasma membrane lipid raft system. The phosphorylated intracellular domains of the receptors are accessible for cytoplasmic Syk tyrosine kinase, which is activated by Lyn-mediated phosphorylation and which transduces the signal to downstream adaptors. Lyn is abnormally distributed in acute myeloid leukemia cells and seems to be a novel pharmacologic target of this disease.
Pathways Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling, Integrin Complex
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN) (AA 8-238) antibody (ABIN94427) Western Blotting analysis (non-reducing conditions) of Lyn using anti-Lyn (LYN-01). L...
Product cited in: Queval, Nicolas, Beau: "Role of Src kinases in mobilization of glycosylphosphatidylinositol-anchored decay-accelerating factor by Dr fimbria-positive adhering bacteria." in: Infection and immunity, Vol. 79, Issue 7, pp. 2519-34, 2011 (PubMed).

Amoui, Dráberová, Tolar, Dráber: "Direct interaction of Syk and Lyn protein tyrosine kinases in rat basophilic leukemia cells activated via type I Fc epsilon receptors." in: European journal of immunology, Vol. 27, Issue 1, pp. 321-8, 1997 (PubMed).

Dráberová, Amoui, Dráber: "Thy-1-mediated activation of rat mast cells: the role of Thy-1 membrane microdomains." in: Immunology, Vol. 87, Issue 1, pp. 141-8, 1996 (PubMed).