Neurofilament Medium Protein antibody

Details for Product No. ABIN94435
Clonality (Clone)
Monoclonal ()
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Immunogen Pellet of porcine brain cold stable proteins after depolymerization of microtubules.
Clone NF-09
Isotype IgG2a
Specificity The antibody NF-09 reacts with both phosphorylated and non-phosphorylated form of medium neurofilament protein (160 kDa) of various species.
Cross-Reactivity (Details) Mammalian
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Background Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights –, neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Neurofilament Medium Protein antibody (ABIN94435) Western blotting analysis of neurofilament medium protein in porcine brain lysate (re...
Image no. 2 for anti-Neurofilament Medium Protein antibody (ABIN94435) Immunofluorescence staining of neurofilament medium protein in murine Neuro2A cells b...
Product cited in: Dráberová, Sulimenko, Kukharskyy, Dráber: "Monoclonal antibody NF-09 specific for neurofilament protein NF-M." in: Folia biologica, Vol. 45, Issue 4, pp. 163-5, 2000 (PubMed).