NEFM antibody (AA 105-287)

Catalog No. ABIN7433474

Product Details anti-NEFM Antibody

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Binding Specificity: AA 105-287

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEFM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Purpose: Polyclonal Antibody to Neurofilament 3 (NEF3)

Specificity: The antibody is a rabbit polyclonal antibody raised against NEF3. It has been selected for its ability to recognize NEF3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Neurofilament 3 (NEF3) corresdonding to Leu105~Gln287 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:320-1300 Immunohistochemistry: 5-20 μg/mL

1:32-130 Immunocytochemistry: 5-20 μg/mL

1:32-130 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for NEFM

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Alternative Name: Neurofilament 3 (NEFM Products)

Synonyms: NEF3 antibody, NF-M antibody, NFM antibody, Nef3 antibody, Nfm antibody, NF160 antibody, NF165 antibody, nef-m antibody, nef3 antibody, nefm antibody, nf-m antibody, zgc:112359 antibody, nefm-a antibody, nefm-b antibody, neurofilament medium antibody, neurofilament triplet M protein antibody, neurofilament, medium polypeptide antibody, neurofilament medium polypeptide antibody, neurofilament medium L homeolog antibody, neurofilament, medium polypeptide a antibody, neurofilament medium S homeolog antibody, NEFM antibody, Nefm antibody, TVAG_582010 antibody, TVAG_379630 antibody, nefm.L antibody, nefma antibody, nefm.S antibody

Background: NEFM, NF-M, NFM, Neurofilament,Medium Polypeptide, 160 kDa neurofilament protein, Neurofilament triplet M protein

Pathways: Brown Fat Cell Differentiation