Angiopoietin 1 antibody (ANGPT1)

Details for Product anti-ANGPT1 Antibody No. ABIN95109
Antigen
Reactivity
Mouse (Murine)
168
72
59
24
17
10
5
3
2
1
1
1
1
1
1
1
1
1
Host
Rabbit
186
13
7
1
Clonality
Polyclonal
Conjugate
This Angiopoietin 1 antibody is un-conjugated
24
11
7
4
4
4
4
4
4
4
4
3
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
160
74
55
27
16
13
13
10
8
6
6
5
2
2
Options
Immunogen This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide N-Q-R-R-N-P-E-N-G-G-R-R-Y-N-R-I-Q-H-G-Q corresponding to a region (aa 21-40) near the of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Characteristics Concentration Definition: by Refractometry
Antigen
Alternative Name Angiopoietin 1 (ANGPT1 Antibody Abstract)
Background Anti Antiopoietin-1 Antibody recognizes Angiopoietin-1 (Ang-1) that has importance in the development of the endothelium by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has a potential fibrinogen-like domain at the carboxyl terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, Ang-1 is also expressed in the heart and liver.
Synonyms: AGP 1 antibody, AGP1 antibody, AGPT antibody, ANG 1 antibody, ANG1 antibody, Angiopoietin1 antibody, ANGPT 1 antibody, ANGPT1 antibody, KIAA0003 antibody
Gene ID 11600, 46048213
NCBI Accession NP_031452
UniProt O08538
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process
Application Notes Suitable for western blotting, Immunohistochemistry (IHC) and other antibody based assays.  A 1:500 dilution is recommended for western blotting.  The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum.  This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in a very clean and strong signal.  Both Ang-1 and Ang-2 proteins have predicted molecular weights of approximately 57 kDa and appear on western blots close to their predicted molecular weights.  In some instances additional bands may be seen at approximately 75 kDa which represent highly glycosylated forms of the protein that migrate at a higher apparent molecular weight.
Restrictions For Research Use only
Format Liquid
Concentration 85 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-Angiopoietin 1 (ANGPT1) antibody (ABIN95109) Rabbit anti-Ang-1 was used at a 1:500 dilution to detect mouse Ang-1 by western blot ...
Image no. 2 for anti-Angiopoietin 1 (ANGPT1) antibody (ABIN95109) anti-ANG1 antibody was diluted 1:500 to detect ANG1 in human lung tissue. Tissue was ...
Product cited in: Egginton, Hussain, Hall-Jones, Chaudhry, Syeda, Glen: "Shear stress-induced angiogenesis in mouse muscle is independent of the vasodilator mechanism and quickly reversible." in: Acta physiologica (Oxford, England), Vol. 218, Issue 3, pp. 153-166, 2017 (PubMed).

Dallabrida, Ismail, Pravda, Parodi, Dickie, Durand, Lai, Cassiola, Rogers, Rupnick: "Integrin binding angiopoietin-1 monomers reduce cardiac hypertrophy." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 22, Issue 8, pp. 3010-23, 2008 (PubMed).

Câmpean, Karpe, Haas, Atalla, Peters, Rupprecht, Liebner, Acker, Plate, Amann: "Angiopoietin 1 and 2 gene and protein expression is differentially regulated in acute anti-Thy1.1 glomerulonephritis." in: American journal of physiology. Renal physiology, Vol. 294, Issue 5, pp. F1174-84, 2008 (PubMed).