APG8 antibody

Details for Product No. ABIN95223
Antigen
Reactivity
Yeast (Saccharomyces cerevisiae)
Host
Rabbit
Clonality
Polyclonal
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Options
Immunogen This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast APG8 protein.
Immunogentype:Recombinant
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Antigen
Background Anti-ATG8 antibody is an Ubiquitin-like protein (UBL) antibody. UBL proteins and antibodies fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin.  Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12.  Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs).  These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other.  In contrast to UBLs, UDPs are not conjugated to other proteins.  Apg8 is required for autophagy (intracellular bulk protein degradation) in yeast.  Starved yeast cells take up their own cytoplasm into vacuoles through autophagic bodies.   Autophagic bodies form a double-membraned structure called the autophagosome, which subsequently fuses with the vacuole/lysosome. This process similar in mammals.  Two sets of genes, APG and AUT, have been identified with this process, and are responsible for two ubiquitin-like systems Apg12 and Apg8, respectively.  Apg12 is synthesized in its mature form and seems to have one target, Apg5.  Almost all Apg12 molecules are conjugated with Apg5.  Aut2/Apg4 processes the Apg8/Aut7 system at its carboxy-terminal region.  Apg8 exists in two forms, one is membrane bound through a phospholipid. Lipidation/ activation of Apg8 is mediated by Apg7 and transferred to Apg3 and finally forms a conjugate with phosphatidyl-ethanolamine (PE). Apg4 cleaves Apg8–PE, releasing Apg8 from membrane.  Morphological studies show that Apg8 localizes on the membrane of intermediate structures of the autophagosome; this transient association seems to be essential for formation of the autophagosome. Both Apg12 and Apg8 are highly conserved, with apparent homologues in the worm, mammals and plants. In higher eukaryotes, Apg8 consists of a multigene family. Useful in Cell Biology, Microbiology and Signal Transduction research.
Synonyms: ATG8 Antibody, Autophagy-related protein 8, Autophagy-related ubiquitin-like modifier ATG8, Cytoplasm to vacuole targeting protein 5
Gene ID 852200, 6319393
UniProt P38182
Application Notes This purified polyclonal antibody reacts with yeast APG8 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.   This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates).  Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions. For immunoblotting a 14 kDa band corresponding to yeast APG8 is detected.  Most yeast cell lysates can be used as a positive control without induction or stimulation. 
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 5.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
Image no. 1 for anti-APG8 antibody (ABIN95223) Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifier...
Image no. 2 for anti-APG8 antibody (ABIN95223) Western blot of APG8 fusion protein. Anti-APG8 antibody generated by immunization wit...