(1 reference)
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- Target See all Biotin products
- Biotin
- Reactivity
- Please inquire
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Biotin antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Supplier Product No.
- 600-101-098
- Supplier
- Rockland
- Purpose
- Biotin Antibody
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
- Characteristics
- Synonyms: goat anti biotin antibody
- Purification
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
Immunogen Type: Other
- Isotype
- IgG
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anti-Biotin antibody
WB, ELISA, FACS, IHC, IF, FM Host: Mouse Monoclonal 4C7-D5 unconjugated
anti-Biotin antibodyWB, FACS, IHC, IF, ISt, CM Host: Mouse Monoclonal Hyb-8 unconjugated
anti-Biotin antibodyIHC, ISt Host: Rabbit Monoclonal BTN-2032R unconjugated Recombinant Antibody
anti-Biotin antibodyWB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Biotin antibody (DyLight 488)WB, FACS, IF, FM, FLISA, DB, MA Host: Goat Polyclonal DyLight 488
anti-Biotin antibodyELISA, FACS Host: Mouse Monoclonal SB58c unconjugated
anti-Biotin antibodyELISA, FACS, IP, CyTOF Host: Mouse Monoclonal 1D4-C5 unconjugated
anti-Biotin antibodyReactivity: Human WB Host: Rabbit Monoclonal unconjugated
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- Application Notes
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Biotin has been tested by ELISA is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Optimal concentrations in other immunoassays should be determined by the researcher.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:20,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.02 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- This product is stable for several weeks at 4° C as an undiluted liquid. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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: "Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures." in: Nature communications, Vol. 9, Issue 1, pp. 3415, (2018) (PubMed).
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: "Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures." in: Nature communications, Vol. 9, Issue 1, pp. 3415, (2018) (PubMed).
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- Target
- Biotin
- Abstract
- Biotin Products
- Target Type
- Chemical
- Background
- Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
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