NFATC1 antibody

Catalog No. ABIN967559

Product Details anti-NFATC1 Antibody

Target: NFATC1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NFATC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Brand: BD Pharmingen™

Cross-Reactivity: Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: 7A6

Isotype: IgG1 kappa

Application Details

Application Notes: 7A6 may be used for western blot analysis of NF-ATc1 (1-2 µg/ml). Note that while the predicted M W of NF-ATc is ~100 kDa, the observed M W may range between 100 to 120 kDa due to post-translational modification of NF-ATc. Other applications include immunoprecipitation (2 µg/ml), mobility shift assays (EMSA) and immunofluorescence microscopy of paraformaldehyde-fixed, tissue cultured cells. Jurkat human T cells (ATCC TIB-152) are suggested as a positive control for expression of NF-ATc.

Comment:

Related Products: ABIN967389, ABIN968537

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-NFATC1 antibody (ABIN967559)

Yoshida, Nishina, Takimoto, Marengère, Wakeham, Bouchard, Kong, Ohteki, Shahinian, Bachmann, Ohashi, Penninger, Crabtree, Mak: "The transcription factor NF-ATc1 regulates lymphocyte proliferation and Th2 cytokine production." in: Immunity, Vol. 8, Issue 1, pp. 115-24, (1998) (PubMed).

Timmerman, Healy, Ho, Chen, Goodnow, Crabtree: "Redundant expression but selective utilization of nuclear factor of activated T cells family members." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 6, pp. 2735-40, (1997) (PubMed).

Timmerman, Clipstone, Ho, Northrop, Crabtree: "Rapid shuttling of NF-AT in discrimination of Ca2+ signals and immunosuppression." in: Nature, Vol. 383, Issue 6603, pp. 837-40, (1996) (PubMed).

Northrop, Ho, Chen, Thomas, Timmerman, Nolan, Admon, Crabtree: "NF-AT components define a family of transcription factors targeted in T-cell activation." in: Nature, Vol. 369, Issue 6480, pp. 497-502, (1994) (PubMed).

Crabtree: "Contingent genetic regulatory events in T lymphocyte activation." in: Science (New York, N.Y.), Vol. 243, Issue 4889, pp. 355-61, (1989) (PubMed).

Target Details for NFATC1

Target: NFATC1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1))

Alternative Name: NF-ATc1 (NFATC1 Products)

Background: The NF-AT family of transcription factors are regulators of early immune response genes in T cells following their activation by CD40L, FasL and nterleukins including IL-2, -3, -4 and -5. NF-AT proteins function in both signal transduction and transcription control. The DNA binding, active NF-AT complex contains a cytoplasmic, Ca2+/calcineurin dependent, cyclosporin sensitive subunit, designated NF-ATc, which is present in the cytoplasm of resting cells. Upon cell activation, NF-ATc is dephosphorylated and thus activated by calcineurin, resulting in rapid translocation of NF-ATc to the nucleus. The active NF-AT complex also contains a nuclear component, NF-ATn. In vitro, NF-AT cooperates with the mitogenic transcription factor AP-1 to induce multiple cytokine genes. NF-ATc is encoded by four genes: NF-ATc1 (originally named NF-ATc), NF-ATc2 (originally named NF-ATp), NF-ATc3 (NF-AT3) and NF- ATc4 (NF-AT4). These proteins are differentially expressed in tissues, suggesting that each may activate distinct sets of genes. NF-ATc1 is expressed in lymphoid organs and is upregulated in activated T cell and thymocytes. NF-ATc1 is required for the development of T cells and has been shown to contribute to the production of IL-2 and the development of Th2 responses. 7A6 recognizes human and mouse NF-ATc1. The antibody recognizes a region between amino acids 197 to 304 of human NF-ATc1, which lies outside the region of homology between NF-ATc1 and NF-ATc2.3 Please note that in the original publication for the antibody (Northrop et al. 1994), the clone name 7A6 is not specifically identified.

Molecular Weight: 100-120 kDa

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway