CREB1 antibody (cAMP Responsive Element Binding Protein 1) (AA 122-147)

Details for Product anti-CREB1 Antibody No. ABIN97863
Antigen
Epitope
AA 122-147, pSer133
123
45
40
30
20
18
16
15
10
10
9
9
8
7
6
6
5
5
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
573
358
325
42
35
32
31
26
22
16
15
13
13
6
6
5
2
1
1
1
1
Host
Rabbit
508
72
7
1
Clonality
Polyclonal
Conjugate
This CREB1 antibody is un-conjugated
26
15
15
13
13
13
13
13
13
8
7
6
3
3
3
3
3
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
434
236
223
90
89
50
44
44
23
14
11
10
4
4
2
2
1
1
Options
Immunogen CREB phospho peptide corresponding to amino acid residues 122-147 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Antigen
Alternative Name CREB (CREB1 Antibody Abstract)
Background The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome. Phosphorylation by cAMP-dependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II.  Alternate splicing of this gene results in two transcript variants encoding different isoforms.
Synonyms: Active transcription factor CREB antibody, cAMP response element binding protein antibody, cAMP responsive element binding protein 1 antibody, CREB 1 antibody, CREB1 antibody, MGC9284 antibody, Transactivator protein antibody
Gene ID 1385
UniProt P16220
Pathways TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation
Application Notes This phospho specific polyclonal antibody reacts with phosphorylated human CREB at pS133 and shows minimal reactivity by western blot with non-phosphorylated CREB and minimal reactivity (1%) by ELISA against the non-phosphorylated form of the immunizing peptide.  This antibody was assayed against a variety of tissues including fibroblasts and B-cell lysates.  Bands of 46 and 43 kDa corresponding to phosphorylated CREB are observed in western blots.  Anti-CREB pS133 is suitable for the detection by immunoblot of phosphoryl-ated human, mouse and rat CREB.  No cross-reactivity occurs with non-phosphorylated CREB.  For immunohistochemistry, formalin fixed, paraffin embedded human tissue shows moderate to strong nuclear staining in a variety of cells with minimal background staining.  Although not tested this antibody is likely functional by FACS and immunoprecipitation.
Restrictions For Research Use only
Format Liquid
Concentration 0.064 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-cAMP Responsive Element Binding Protein 1 (CREB1) (AA 122-147), (pSer133) antibody (ABIN97863) Anti-CREB pS133 was used to detect phosphorylated CREB by western blot. Recombinant H...
Image no. 2 for anti-cAMP Responsive Element Binding Protein 1 (CREB1) (AA 122-147), (pSer133) antibody (ABIN97863) affinity purified anti-CREB pS133 antibody was used at 20 ?g/ml to detect signal in a...
Image no. 3 for anti-cAMP Responsive Element Binding Protein 1 (CREB1) (AA 122-147), (pSer133) antibody (ABIN97863) Anti-CREB pS133 was used to detect phosphorylated CREB by western blot.  Recombinant ...
Product cited in: Philp, Chen, Lan, Meyer, Murphy, Knapp, Olfert, McCurdy, Marcotte, Hogan, Baar, Schenk et al.: "Sirtuin 1 (SIRT1) deacetylase activity is not required for mitochondrial biogenesis or peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) deacetylation following ..." in: The Journal of biological chemistry, Vol. 286, Issue 35, pp. 30561-70, 2011 (PubMed).