CREB1 antibody (N-Term)

Catalog No. ABIN97864

Product Details anti-CREB1 Antibody

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CREB1 antibody is un-conjugated

Application: Western Blotting (WB)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: CREB-1 (p43) peptide corresponding to a region near the of the human protein, conjugated to Keyhole Limpet Hemocyanin (KLH).

Application Details

Application Notes: This product was assayed against a lysate of 1 x 10^6 Raji B cells by immunoblot and found to be reactive against CREB-1 (p43), showing a 46 kDa band at a dilution of 1:500 to 1:1,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG (H&L) [Goat] Anti-CREB-1 (p43) is suitable for detection by immunoblot of human, mouse and rat CREB-1 (p43). This product was also tested in a gel supershift assay and found to be reactive against CREB-1 (p43) using 0.5 to 1.0 µl per assay. 

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.56 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-CREB1 antibody (ABIN97864)

Chen, Garvin, Nickola, Watson, Colberg-Poley, Rose: "IL-1β induction of MUC5AC gene expression is mediated by CREB and NF-κB and repressed by dexamethasone." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 306, Issue 8, pp. L797-807, (2014) (PubMed).

Wierda, Kuipers, van Eggermond, Benard, van Leeuwen, Carluccio, Geutskens, Jukema, Marquez, Quax, van den Elsen: "Epigenetic control of CCR5 transcript levels in immune cells and modulation by small molecules inhibitors." in: Journal of cellular and molecular medicine, Vol. 16, Issue 8, pp. 1866-77, (2012) (PubMed).

Wang, Sasaki, Zhou, Matsumura, Araki, Mezawa, Takai, Chen, Ogata: "Transcriptional regulation of bone sialoprotein gene by interleukin-11." in: Gene, (2011) (PubMed).

Philp, Chen, Lan, Meyer, Murphy, Knapp, Olfert, McCurdy, Marcotte, Hogan, Baar, Schenk et al.: "Sirtuin 1 (SIRT1) deacetylase activity is not required for mitochondrial biogenesis or peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) deacetylation following ..." in: The Journal of biological chemistry, Vol. 286, Issue 35, pp. 30561-70, (2011) (PubMed).

Target Details for CREB1

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Alternative Name: CREB-1 (CREB1 Products)

Synonyms: creb antibody, CREB1 antibody, Creb1 antibody, CREB antibody, 2310001E10Rik antibody, 3526402H21Rik antibody, AV083133 antibody, Creb antibody, Creb-1 antibody, creb1 antibody, zgc:55598 antibody, cAMP responsive element binding protein 1 antibody, cAMP responsive element binding protein antibody, cAMP responsive element binding protein 1 S homeolog antibody, cAMP responsive element binding protein 1a antibody, CREB1 antibody, creb1 antibody, CREB antibody, Creb1 antibody, creb1.S antibody, creb1a antibody

Background: Anti CREB-1 Antibody recognizes CREB (cAMP response element-binding), a cellular transcription factor. CREB binds to certain DNA sequences called cAMP response elements (CRE), thereby increasing or decreasing the transcription of the downstream genes. CREB was first described in 1987 as a cAMP-responsive transcription factor regulating the somatostatin gene. Genes whose transcription is regulated by CREB include: c-fos, the neurotrophin BDNF (Brain-derived neurotrophic factor), tyrosine hydroxylase, and many neuropeptides (such as somatostatin, enkephalin, VGF, and corticotropin-releasing hormone). CREB is closely related in structure and function to CREM (cAMP response element modulator) and ATF-1 (activating transcription factor-1) proteins. CREB proteins are expressed in many animals, including humans. CREB has a well-documented role in neuronal plasticity and long-term memory formation in the brain.
Synonyms: Cyclic AMP-responsive element-binding protein 1 cAMP-responsive element-binding protein 1 CREB-1 CREB1

Gene ID: 1385

UniProt: P16220

Pathways: TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation