CREB1 antibody (N-Term)
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- Target See all CREB1 Antibodies
- CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))
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Binding Specificity
- N-Term
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CREB1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Gel Shift (GS)
- Supplier Product No.
- 100-401-195
- Supplier
- Rockland
- Purpose
- CREB-1 Antibody
- Characteristics
- Synonyms: rabbit anti-CREB1 Antibody, rabbit anti-p43 antibody, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1, CREB-1, CREB1, p43
- Purification
- Anti-CREB-1 was prepared from monospecific antiserum by delipidation and immunoadsorption against an E.coli lysate immobilized on agarose beads.
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: CREB-1 (p43) peptide corresponding to a region near the N-terminus of the human protein, conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Conjugated Peptide
- Top Product
- Discover our top product CREB1 Primary Antibody
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- Application Notes
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Gel Shift Dilution: 0.5 to 1.0 μLper assay
Application Note: Anti-CREB-1 Antibody has been tested in western blot and suitable in ELISA assays. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:500 - 1:1,000
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 2.56 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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IL-1β induction of MUC5AC gene expression is mediated by CREB and NF-κB and repressed by dexamethasone." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 306, Issue 8, pp. L797-807, (2014) (PubMed).
: "Epigenetic control of CCR5 transcript levels in immune cells and modulation by small molecules inhibitors." in: Journal of cellular and molecular medicine, Vol. 16, Issue 8, pp. 1866-77, (2012) (PubMed).
: "Transcriptional regulation of bone sialoprotein gene by interleukin-11." in: Gene, (2011) (PubMed).
: "Sirtuin 1 (SIRT1) deacetylase activity is not required for mitochondrial biogenesis or peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) deacetylation following ..." in: The Journal of biological chemistry, Vol. 286, Issue 35, pp. 30561-70, (2011) (PubMed).
: "
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IL-1β induction of MUC5AC gene expression is mediated by CREB and NF-κB and repressed by dexamethasone." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 306, Issue 8, pp. L797-807, (2014) (PubMed).
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- Target
- CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))
- Alternative Name
- CREB1 (CREB1 Products)
- Background
- Background: Anti CREB-1 Antibody recognizes CREB (cAMP response element-binding), a cellular transcription factor. CREB binds to certain DNA sequences called cAMP response elements (CRE), thereby increasing or decreasing the transcription of the downstream genes. CREB was first described in 1987 as a cAMP-responsive transcription factor regulating the somatostatin gene. Genes whose transcription is regulated by CREB include: c-fos, the neurotrophin BDNF (Brain-derived neurotrophic factor), tyrosine hydroxylase, and many neuropeptides (such as somatostatin, enkephalin, VGF, and corticotropin-releasing hormone). CREB is closely related in structure and function to CREM (cAMP response element modulator) and ATF-1 (activating transcription factor-1) proteins. CREB proteins are expressed in many animals, including humans. CREB has a well-documented role in neuronal plasticity and long-term memory formation in the brain.
- Gene ID
- 1385
- UniProt
- P16220
- Pathways
- TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation
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