FGA antibody
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- Target See all FGA Antibodies
- FGA (Fibrinogen alpha Chain (FGA))
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This FGA antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Supplier Product No.
- 100-101-240
- Supplier
- Rockland
- Purpose
- Fibrinogen Antibody
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Fibrinogen [Human Plasma].
- Characteristics
- Synonyms: goat anti-Fibrinogen Antibody, FGA antibody, FGA protein antibody, FGB antibody, FGG antibody, Fib2 antibody, Fibrinogen A alpha polypeptide antibody, Fibrinogen A alpha polypeptide chain antibody, Fibrinogen alpha chain antibody
- Purification
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
- Immunogen
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Immunogen: Fibrinogen [Human Plasma]
Immunogen Type: Native Protein
- Top Product
- Discover our top product FGA Primary Antibody
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- Application Notes
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Immunohistochemistry Dilution: 1:500
Application Note: Anti-Fibrinogen has been tested by western blot and immunohistochemistry and is suitable to be assayed against 1.0 μg of Fibrinogen [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:20,000 - 1:100,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 90 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- FGA (Fibrinogen alpha Chain (FGA))
- Alternative Name
- FGA (FGA Products)
- Background
- Background: Fibrinogen is cleaved by the protease thrombin to yield monomers which, together with fibrinogen alpha (FGA), fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, it functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. It was originally thought to be essential for platelet aggregation, based on in vitro studies using anti-coagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. It enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. It may also facilitate the immune response via both innate and T-cell mediated pathways.
- Gene ID
- 2243
- NCBI Accession
- NP_000499
- UniProt
- P02671
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